Production of a Novel Extracellular Polysaccharide by Lactobacillus sake 0-1 and Characterization of the Polysaccharide.

Applied and Environmental Microbiology
D J van den BergC T Verrips

Abstract

A novel exopolysaccharide (EPS) produced by Lactobacillus sake 0-1 (CBS 532.92) has been isolated and characterized. When the strain was grown on glucose, the produced EPS contained glucose and rhamnose in a molar ratio of 3:2 and the average molecular mass distribution (M(infm)) was determined at 6 x 10(sup6) Da. At a concentration of 1%, the 0-1 EPS had better viscosifying properties than xanthan gum when measured over a range of shear rates from 0 to 300 s(sup-1), while shear-thinning properties were comparable. Rheological data and anion-exchange chromatography suggested the presence of a negatively charged group in the EPS. Physiological parameters for optimal production of EPS were determined in batch fermentation experiments. Maximum EPS production was 1.40 g (middot) liter(sup-1), which was obtained when L. sake 0-1 had been grown anaerobically at 20(deg)C and pH 5.8. When cultured at lower temperatures, the EPS production per gram of biomass increased from 600 mg at 20(deg)C to 700 mg at 10(deg)C but the growth rate in the exponential phase decreased from 0.16 to 0.03 g (middot) liter(sup-1) (middot) h(sup-1). EPS production started in the early growth phase and stopped when the culture reached the stationary phase. Gr...Continue Reading

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Citations

Jan 3, 2001·Applied and Environmental Microbiology·A RamosH Santos
Sep 21, 2019·Biotechnology and Applied Biochemistry·Bhavana V MohiteSatish V Patil
Dec 30, 1998·FEMS Microbiology Letters·M BreedveldL Dijkhuizen
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Aug 23, 2011·Archives of Microbiology·Ann K StaudtJoshua D Shrout
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Feb 2, 2013·Iranian Journal of Environmental Health Science & Engineering·Mostafa Mohamed El-SheekhRania El-Shenody
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Myriam LoefflerJochen Weiss
Dec 5, 2018·Food Science & Nutrition·Oniovosa Leonard Adamu-GovernorSylvia Veronica Ajagugha Uzochukwu
Jun 3, 1998·Applied and Environmental Microbiology·D LowJ R Broadbent
Nov 5, 1999·Applied and Environmental Microbiology·P J LooijesteijnJ Hugenholtz

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