PMID: 9435221Jan 22, 1998Paper

Production of a polyketide natural product in nonpolyketide-producing prokaryotic and eukaryotic hosts

Proceedings of the National Academy of Sciences of the United States of America
J T KealeyP J Barr

Abstract

The polyketides are a diverse group of natural products with great significance as human and veterinary pharmaceuticals. A significant barrier to the production of novel genetically engineered polyketides has been the lack of available heterologous expression systems for functional polyketide synthases (PKSs). Herein, we report the expression of an intact functional PKS in Escherichia coli and Saccharomyces cerevisiae. The fungal gene encoding 6-methylsalicylic acid synthase from Penicillium patulum was expressed in E. coli and S. cerevisiae and the polyketide 6-methylsalicylic acid (6-MSA) was produced. In both bacterial and yeast hosts, polyketide production required coexpression of 6-methylsalicylic acid synthase and a heterologous phosphopantetheinyl transferase that was required to convert the expressed apo-PKS to its holo form. Production of 6-MSA in E. coli was both temperature- and glycerol-dependent and levels of production were lower than those of P. patulum, the native host. In yeast, however, 6-MSA levels greater than 2-fold higher than the native host were observed. The heterologous expression systems described will facilitate the manipulation of PKS genes and consequent production of novel engineered polyketides a...Continue Reading

References

Dec 15, 1992·The Biochemical Journal·J B Spencer, P M Jordan
Mar 1, 1992·Molecular & General Genetics : MGG·I LuqueF Madueño
Dec 1, 1981·Journal of Bacteriology·S Jackowski, C O Rock
Apr 1, 1994·Bio/technology·C R Hutchinson
Dec 3, 1993·Science·R McDanielC Khosla
Nov 7, 1996·Nature·G L Verdine
Nov 1, 1996·Chemistry & Biology·R H LambalotC T Walsh
Jun 1, 1990·Journal of Chemical Ecology·J L SeidelD W Davidson

❮ Previous
Next ❯

Citations

Jun 27, 2007·Chembiochem : a European Journal of Chemical Biology·Xi ChenPeng George Wang
Jun 5, 2004·Applied Microbiology and Biotechnology·Eduardo RodriguezLeonard Katz
Dec 17, 2009·Applied Microbiology and Biotechnology·Patrick GovenderFlorian F Bauer
Aug 3, 2010·Applied Microbiology and Biotechnology·Florian F BauerMichael C Bester
Apr 1, 2005·Molecular Genetics and Genomics : MGG·P LuJ S Tkacz
Jan 25, 2008·Journal of Industrial Microbiology & Biotechnology·L DuQ Wang
Aug 22, 2001·Trends in Biotechnology·V MiaoJ Davies
Sep 30, 1998·Current Opinion in Biotechnology·C W Carreras, D V Santi
Oct 6, 2001·Current Opinion in Microbiology·E Rodriguez, R McDaniel
Mar 6, 1999·Current Opinion in Microbiology·C R Hutchinson
Mar 6, 2010·The Journal of Antibiotics·Isao Fujii
Aug 12, 2008·Nature Chemical Biology·Kristy M Hawkins, Christina D Smolke
Dec 30, 2010·Nature Reviews. Microbiology·Marnix H MedemaEriko Takano
Nov 23, 2007·Natural Product Reports·J Stefan RokemJens Nielsen
Jun 3, 2005·Genome Research·Irina BorodinaJens Nielsen
Aug 19, 2008·Applied and Environmental Microbiology·Patrick GovenderFlorian F Bauer
Jan 27, 2009·Applied and Environmental Microbiology·Gianni PanagiotouLisbeth Olsson
May 7, 2004·Applied and Environmental Microbiology·Stephane GrazianiMarie-Josee Daboussi
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·B A Pfeifer, C Khosla
Jan 1, 2011·Annual Review of Chemical and Biomolecular Engineering·Lauren B PickensYit-Heng Chooi
Jun 29, 2000·Annual Review of Biochemistry·C KhoslaD E Cane
Aug 8, 2008·Microbial Cell Factories·Beate Pscheidt, Anton Glieder
May 24, 2003·Applied Microbiology and Biotechnology·R RegentinP Licari
Oct 23, 2013·Journal of Industrial Microbiology & Biotechnology·Philipp Wiemann, Nancy P Keller
Aug 1, 2014·Journal of Biotechnology·Jin Wook Choi, Nancy A Da Silva
Nov 17, 2006·Nature Chemical Biology·Michelle C Y Chang, Jay D Keasling
Apr 30, 2013·Journal of the American Chemical Society·Yi-Ming ChiangBerl R Oakley
Feb 7, 2016·Fungal Genetics and Biology : FG & B·Carly BondLi Li
Jan 26, 2016·Fungal Genetics and Biology : FG & B·Yong Fuga LiLouise K Charkoudian
Dec 30, 2014·Current Opinion in Biotechnology·Anastasia Krivoruchko, Jens Nielsen
Nov 24, 2012·Fungal Genetics and Biology : FG & B·Juntira PunyaSupapon Cheevadhanarak
Jan 17, 2012·Current Opinion in Biotechnology·Satoshi YuzawaJay D Keasling
Oct 5, 2011·Biotechnology Advances·Owen P Ward
Mar 5, 2016·Current Opinion in Biotechnology·Mahmoud Kamal Ahmadi, Blaine A Pfeifer
Jun 30, 2009·Metabolic Engineering·Wenjuan ZhaHuimin Zhao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.