Production of a recombinant swollenin from Trichoderma harzianum in Escherichia coli and its potential synergistic role in biomass degradation

Microbial Cell Factories
Clelton A SantosAnete Pereira de Souza

Abstract

Fungal swollenins (SWOs) constitute a class of accessory proteins that are homologous to canonical plant expansins. Expansins and expansin-related proteins are well known for acting in the deagglomeration of cellulose structure by loosening macrofibrils. Consequently, SWOs can increase the accessibility and efficiency of the other enzymes involved in the saccharification of cellulosic substrates. Thus, SWOs are promising targets for improving the hydrolysis of plant biomass and for use as an additive to enhance the efficiency of an enzyme cocktail designed for the production of biofuels. Here, we report the initial characterization of an SWO from Trichoderma harzianum (ThSwo) that was successfully produced using Escherichia coli as a host. Initially, transcriptome and secretome data were used to compare swo gene expression and the amount of secreted ThSwo. The results from structural modeling and phylogenetic analysis of the ThSwo protein showed that ThSwo does preserve some structural features of the plant expansins and family-45 glycosyl hydrolase enzymes, but it evolutionarily diverges from both of these protein classes. Recombinant ThSwo was purified at a high yield and with high purity and showed secondary folding similar ...Continue Reading

References

Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·D J CosgroveD M Durachko
Oct 3, 2000·Nature·D J Cosgrove
Oct 29, 2000·Journal of Biotechnology·C MawadzaB Mattiasson
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Jan 1, 2004·Nature·Ling QinJohannes Helder
Sep 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Neela H YennawarDaniel J Cosgrove
Mar 17, 2009·Applied Biochemistry and Biotechnology·Mingjia ZhangZhimin He
Feb 25, 2010·Biotechnology for Biofuels·Valdeir Arantes, Jack N Saddler
May 7, 2010·Applied Biochemistry and Biotechnology·Meihua WangZhinan Xu
Sep 28, 2010·Critical Reviews in Biotechnology·Mehdi DashtbanWensheng Qin
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Oct 24, 2013·Molecular Biology and Evolution·Nikolas NikolaidisDaniel J Cosgrove
Feb 22, 2014·PloS One·Maria Augusta Crivelente HortaAnete Pereira Souza
Oct 11, 2014·Nucleic Acids Research·Ivica LetunicPeer Bork
Mar 25, 2015·PloS One·Omar Eduardo Tovar-HerreraJorge Luis Folch-Mallol
Apr 3, 2015·Applied Microbiology and Biotechnology·Nikolaos GeorgelisDaniel J Cosgrove
Jun 9, 2015·PloS One·Gustavo Pagotto BorinJuliana Velasco de Castro Oliveira
Jun 10, 2015·Current Opinion in Plant Biology·Daniel J Cosgrove
Dec 19, 2015·Current Protocols in Bioinformatics·Jianyi Yang, Yang Zhang
Feb 11, 2016·Biochemical Society Transactions·Glyn R HemsworthHarry Brumer
Mar 19, 2016·Biotechnology for Biofuels·Willian Daniel Hahn SchneiderMarli Camassola
May 24, 2016·Trends in Biochemical Sciences·Vijai K GuptaThaddeus Ezeji

❮ Previous
Next ❯

Datasets Mentioned

BETA
KM555252.1

Methods Mentioned

BETA
size-exclusion chromatography
circular dichroism
light microscopy
scanning electron microscopy
PCR
gel filtration

Software Mentioned

CDNN
Origin
BLAST
PYMOL
ClustalW
Avicel
TASSER
MEGA6
ThSwo
FigTree

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.