Production of a therapeutic protein by fusing it with two fragments of the carboxyl-terminal peptide of human chorionic gonadotropin β-subunit in Pichia pastoris

Biotechnology Letters
Baolong WangMeiqing Feng

Abstract

To produce a therapeutic protein (endostatin) by fusion with two fragments of the carboxyl-terminal peptide (CTP) of the human chorionic gonadotropin β-subunit in Pichia pastoris. Two CTP sequences were fused to the C-terminal of human endostatin, and the fusion protein (endo-CTP) was expressed by P. pastoris. Endo-CTP inhibited proliferation of endothelial cells with an IC50 of 7 μg ml(-1), and 30 % of cells were annexin V-positive after treatment with 20 μg endo-CTP ml(-1) for 48 h. Migration of endothelial cells was inhibited by endo-CTP in a concentration-dependent manner. The half-life of endo-CTP in Sprague-Dawley rats was much longer than that of its commercial counterpart (Endostar). A long-acting endostatin can be produced using CTP technology.

References

Apr 17, 1999·The Journal of Biological Chemistry·M DhanabalV P Sukhatme
Jun 10, 2008·Current Protein & Peptide Science·Huan-Li XuWei Tang
Feb 5, 2010·Journal of Cancer Research and Clinical Oncology·Guichun Huang, Longbang Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.