PMID: 8943070Nov 26, 1996Paper

Production of active bovine tracheal antimicrobial peptide in milk of transgenic mice

Proceedings of the National Academy of Sciences of the United States of America
S YarusG Diamond

Abstract

Tracheal antimicrobial peptide (TAP) is a member of the beta-defensin family of antibiotic peptides found in the tracheal mucosa of the cow. TAP gene expression in the bovine airway is inducible by lipopolysaccharide and inflammatory mediators, suggesting that it functions to protect the upper airway from infection. Limited availability of bovine TAP (bTAP) has precluded investigation of its potential utility in agriculture and medicine. To overcome this problem, transgenic mice expressing bTAP using an expression vector driven by control sequences from the murine whey acidic protein (WAP) gene have been generated. The WAP/bTAP transcript was detected in RNA isolated from mammary tissue of transgenic females. bTAP was purified to homogeneity from milk via acid precipitation, reverse-phase HPLC, and ion-exchange chromatography. This milk-derived bTAP had antimicrobial activity against Escherichia coli. Amino-terminal peptide sequencing confirmed the identity of this material as a bTAP isoform. bTAP available from a mammary gland bioreactor will allow evaluation of bTAP for use as an antibiotic in agriculture and medicine.

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Citations

Apr 1, 1999·Biotechnology and Bioengineering·C HaughtR G Harrison
Sep 23, 1998·Clinical Immunology and Immunopathology·G Diamond, C L Bevins
Apr 26, 2006·Applied Microbiology and Biotechnology·I BrocalA Estepa
Oct 29, 2011·Biochemical and Biophysical Research Communications·Xiang-Yang Miao, Xin Zhang
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Jul 6, 2010·Protein Engineering, Design & Selection : PEDS·Brian C BryksaRickey Y Yada
Mar 8, 2016·Asian-Australasian Journal of Animal Sciences·Minseok SeoHeebal Kim
Sep 12, 2006·International Journal of Antimicrobial Agents·Chunyi YinGeorge T-J Huang
Aug 22, 2013·Microbiology·Johan Arnold VoslooMarina Rautenbach
Nov 22, 2005·Biochemical and Biophysical Research Communications·Chunyi YinGeorge T-J Huang
Apr 14, 2006·Veterinary Research·Pascal Rainard, Céline Riollet
Mar 12, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kentaro NagaokaSenkiti Sakai
Mar 6, 2004·Journal of Animal Science·D E Kerr, O Wellnitz
May 2, 2020·Expert Review of Clinical Pharmacology·Kamila Botelho Sampaio de OliveiraSimoni Campos Dias

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