Production of Adult Human Synovial Fluid-Derived Mesenchymal Stem Cells in Stirred-Suspension Culture

Stem Cells International
Kristen D JorgensonArindom Sen

Abstract

The chondrogenic potential of synovial fluid-derived mesenchymal stem cells (SF-MSCs) supports their use in cartilage regeneration strategies. However, their paucity in synovial fluid necessitates their proliferation in culture to generate clinically relevant quantities. Here it was determined that 125 mL stirred suspension bioreactors utilizing Cytodex-3 microcarrier beads represent a viable platform for the proliferation of these cells. During the inoculation phase, a bead loading of 2 g/L, an inoculation ratio of 4.5 cells/bead, and continuous agitation at 40 rpm in a medium with 5% serum resulted in high cell attachment efficiencies and a subsequent overall cell fold expansion of 5.7 over 8 days. During the subsequent growth phase, periodic addition of new microcarriers and fresh medium increased culture longevity, resulting in a 21.3 cell fold increase over 18 days in the same vessel without compromising the defining characteristics of the cells. Compared to static tissue culture flasks, a bioreactor-based bioprocess requires fewer handling steps, is more readily scalable, and for the same cell production level, has a lower operating cost as it uses approximately half the medium. Therefore, stirred suspension bioreactors i...Continue Reading

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Citations

Jul 29, 2017·Journal of Tissue Engineering and Regenerative Medicine·Madiha KhurshidArindom Sen
Nov 8, 2018·Stem Cells International·Kar Wey YongArindom Sen
Oct 30, 2020·ACS Applied Materials & Interfaces·Eleana SomvilleKarine Glinel
May 14, 2020·The Journal of Knee Surgery·William FangC Thomas Vangsness

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Methods Mentioned

BETA
biopsy
flow cytometry

Software Mentioned

CellQuest

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