Production of aflatoxin by an Aspergillus flavus isolate cultured under a limited oxygen supply.

Applied and Environmental Microbiology
G ClevströmK Tideman

Abstract

In a previous experiment on the preservation of hay of high moisture content with formic acid, among other agents, aflatoxin was formed in the hay, and aflatoxin-forming strains of Aspergillus flavus were isolated from this hay after incubation in air as well as in an anaerobic jar. One isolate from the anaerobic jar was cultivated in a chemostat (Bioflo model C 30; New Brunswick Scientific Co.) in a defined medium with added B vitamins, yeast extract, or formic acid, with or without gas flow (air or nitrogen). In all cases where spore germination occurred, aflatoxin was formed in the cultures with gas flow, and small quantities of aflatoxins B1 and B2 occurred even in an atmosphere of nitrogen. Addition of B vitamins and supply of traces of air gave an approximately 15-fold increase in the amount of aflatoxin in 2 days. Carbon dioxide enrichment hindered aflatoxin formation on the defined medium even in the presence of B vitamins, but when formic acid was added, small quantities (5 to 15 micrograms/liter) were formed, and this low level remained constant until the gas flow was started.

References

Sep 1, 1971·Applied Microbiology·T V ReddyT A Venkitasubramanian
Mar 1, 1971·Journal of General Microbiology·R W Detroy, A Ciegler
Dec 1, 1980·Applied and Environmental Microbiology·A SharmaG B Nadkarni
Jul 1, 1961·Journal of Bacteriology·S Bartnicki-Garcia, W J Nickerson

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Citations

Jan 1, 1988·The Journal of Applied Bacteriology·J L BlancoG Suárez
Mar 1, 1988·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·J L BlancoG Suárez
Dec 1, 1985·Mycopathologia·G Clevström, H Ljunggren
Sep 1, 1995·Mycotoxin Research·M E GarciaG Suarez
Jul 9, 2014·Applied and Environmental Microbiology·Konstantinos GrintzalisChristos D Georgiou
Sep 25, 2007·Applied and Environmental Microbiology·Ludmila V RozeJohn E Linz
Feb 6, 2020·Frontiers in Microbiology·Rebecca R Sweany, Kenneth E Damann
Nov 18, 2008·Fungal Genetics and Biology : FG & B·D Ryan Georgianna, Gary A Payne

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