Production of alkanes from CO2 by engineered bacteria

Biotechnology for Biofuels
Tapio LehtinenVille Santala

Abstract

Microbial biosynthesis of alkanes is considered a promising method for the sustainable production of drop-in fuels and chemicals. Carbon dioxide would be an ideal carbon source for these production systems, but efficient production of long carbon chains from CO2 is difficult to achieve in a single organism. A potential solution is to employ acetogenic bacteria for the reduction of CO2 to acetate, and engineer a second organism to convert the acetate into long-chain hydrocarbons. In this study, we demonstrate alkane production from CO2 by a system combining the acetogen Acetobacterium woodii and a non-native alkane producer Acinetobacter baylyi ADP1 engineered for alkane production. Nine synthetic two-step alkane biosynthesis pathways consisting of different aldehyde- and alkane-producing enzymes were combinatorically constructed and expressed in A. baylyi. The aldehyde-producing enzymes studied were AAR from Synechococcus elongatus, Acr1 from A. baylyi, and a putative dehydrogenase from Nevskia ramosa. The alkane-producing enzymes were ADOs from S. elongatus and Nostoc punctiforme, and CER1 from Arabidopsis thaliana. The performance of the pathways was evaluated with a twin-layer biosensor, which allowed the monitoring of both ...Continue Reading

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Citations

Sep 25, 2019·Scientific Reports·Solène MoulinFred Beisson
May 1, 2019·Biotechnology and Bioengineering·Milla SalmelaVille Santala
Jun 2, 2020·Metabolic Engineering Communications·Jin LuoSuvi Santala
Mar 27, 2021·Essays in Biochemistry·Suvi Santala, Ville Santala
Jul 19, 2021·Microbiological Research·Ayesha NisarSardar Azhar Mehmood

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Methods Mentioned

BETA
biosensor
biosensors
genetic
PCR

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