Production of anti-methicillin-resistant Staphylococcus activity from Bacillus subtilis sp. strain B38 newly isolated from soil

Applied Biochemistry and Biotechnology
Olfa TabbeneFerid Limam

Abstract

B38 bacterial strain, isolated from Tunisian soil showed a strong antimicrobial activity. Based on biochemical characterization and 16S rDNA sequence analysis, B38 strain was identified as Bacillus subtilis. Cell culture supernatant showed antibacterial activity against clinical isolates of methicillin-resistant Staphylococcus species and several Gram-positive and Gram-negative bacteria. Antifungal activity against phytopathogenic fungi was also observed. Antibacterial activity production started at early exponential growth phase, and maximum activity was reached at the stationary phase. This antibacterial activity was neither affected by proteases, lipase, and organic solvents, nor by surfactants. It was stable over a wide pH range and still active after autoclaving at 121 degrees C during 20 min. Thin layer chromatography followed by bioautography assay allowed the detection of four active spots with R(f) values of 0.30, 0.47, 0.70, and 0.82. The single spot with R (f) 0.30 showed antifungal activity, whereas the spots with R(f) values of 0.47, 0.70, and 0.82 exhibited antibacterial activity.

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Citations

Jul 1, 2010·World Journal of Microbiology & Biotechnology·Sevdalina Todorova, Lubka Kozhuharova
Jan 15, 2010·FEMS Microbiology Letters·Olfa TabbeneFerid Limam
Jan 6, 2011·FEMS Microbiology Letters·Olfa TabbeneFerid Limam
Feb 18, 2012·Natural Product Research·Salem ElkahouiHartmut Laatsch
Sep 27, 2018·Applied Biochemistry and Biotechnology·Siwar SoussiFerid Limam
Mar 24, 2021·Letters in Applied Microbiology·D S BonaldiC F Silva

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