PMID: 6169359Sep 1, 1981Paper

Production of antibodies against antigens released from human pancreatic tumour xenografts

British Journal of Cancer
A G Grant, D Duke

Abstract

Antibodies directed against the antigen released from viable tumour cells during growth have been raised by cross-immunization of immunocompetent hairy litter-mates with serum from nude mice bearing pancreatic tumour xenografts. Antisera raised against the components released from a primary pancreatic tumour xenograft (WB) or from a tumour cell-line xenograft (GER) showed a titre greater than 1:625 against cultured pancreatic tumour cells by an I125-binding assay. Five out of 14 of the hairy litter-mates immunized with serum from the same tumour (GER) produced antisera that bound more strongly to pancreatic cancer cells than to 13 other non-pancreatic cell lines (binding ratio greater than 2). Absorption of the antisera with pure CEA reduced the level of binding by 11-25% without affecting the specificity for pancreatic tumour cells. The antibody response could be completely removed by absorption with pancreatic tumour cells, whereas 50% and 18% of the activity remained after absorption with normal pancreas homogenate and a mixed non-pancreatic tumour-cell pool, respectively. Immunofluorescent staining of pancreatic tumour sections indicated that the antibody was localized on the membrane of ductular epithelial cells. Challenge...Continue Reading

Citations

Jan 1, 1984·Archives of Oto-rhino-laryngology·B J BraakhuisG B Snow
Oct 1, 1982·The British Journal of Surgery·B GreenwayR Williams
Jan 1, 2009·Pancreas·Petros X E MouratidisAngus G Dalgleish
Nov 30, 2006·International Journal of Cancer. Journal International Du Cancer·Petros X E MouratidisAngus G Dalgleish
Apr 16, 1985·Biochemical and Biophysical Research Communications·M HollmannH P Fortmeyer

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