Production of aromatics in Saccharomyces cerevisiae--a feasibility study

Journal of Biotechnology
Jens O KrömerCristian Varela

Abstract

Aromatics are amongst the most important bulk feedstocks for the chemical industry, however, no viable bioprocess exists today and production is still dependent on petro-chemistry. In this article the production of aromatic precursors such as p-hydroxybenzoic acid (PHBA) and p-amino benzoic acid (PABA) in Saccharomyces cerevisiae was evaluated using metabolic network analysis. Theoretical mass yields for PHBA and for PABA obtained by metabolic network analysis were 0.58 and 0.53 g g(glucose)⁻¹, respectively. A major setback for microbial production of aromatics is the high toxicity of the products. Therefore, PHBA and PABA toxicity was evaluated in S. cerevisiae. Minimal inhibitory concentrations of 38.3 g L⁻¹ for PHBA and 0.62 g L⁻¹ for PABA were observed. However, PABA toxicity could be alleviated in adaptation experiments. Finally, metabolic engineering was used to create proof of principle first generation strains of S. cerevisiae. Overall accumulation of 650 μM PHBA and 250 μM PABA could be achieved.

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Citations

Aug 28, 2014·Microbial Cell Factories·Rebekah McKennaDavid R Nielsen
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Daisuke KomaTakashi Ohmoto
May 28, 2016·Microbial Cell Factories·Nils J H AvereschJens O Krömer
Aug 6, 2016·Biotechnology Journal·Bin LaiJens O Krömer
Dec 15, 2016·Frontiers in Bioengineering and Biotechnology·Shiqin YuJens O Krömer
Mar 9, 2018·Applied Microbiology and Biotechnology·Songwei WangXuehong Zhang
Feb 28, 2019·Microbial Cell Factories·Damla HuccetogullariSang Yup Lee
May 22, 2017·Bioprocess and Biosystems Engineering·Nils J H AvereschJens O Krömer
Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Jens O KrömerNorbert Kohlheb
Dec 29, 2017·Journal of Agricultural and Food Chemistry·Pedro LabordaFengquan Liu
Mar 13, 2019·ACS Synthetic Biology·Ramesh K JhaTaraka Dale

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