Production of autoantibodies against citrullinated antigens/peptides by human B cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
Maria F BellatinPeter Stastny

Abstract

Autoantibodies against citrullinated protein Ags (ACPA) are associated with the development of rheumatoid arthritis (RA). This immune response against citrullinated protein Ags, which is thought to be facilitated by certain MHC HLA-DR alleles, is highly specific for this disease and has been speculated to be involved in the pathogenesis. We have previously studied cultures of B cells for the production of Abs against HLA Ags. The aim of the current study was to examine the role of B cells in the production of ACPA in patients with RA. Peripheral blood B cells from RA patients and healthy people were cultured with EL4-B5, a murine cell line expressing human CD40L, and with T cell factors to stimulate the in vitro production of Abs by B cells isolated from peripheral blood. ACPA were produced by cultured B cells from RA patients, as determined by reactivity to cyclic citrullinated peptide (CCP). The results showed that 22% of the healthy persons tested also had B cells that could produce ACPA. Patients with HLA-DR alleles carrying the RA-associated shared epitope appeared to have more B cells with autoimmune potential for CCP than those without such HLA alleles (odds ratio 8.1, p = 0.001). In healthy individuals, anti-CCP-produci...Continue Reading

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Citations

May 17, 2014·Rheumatology·Elizabeth PerryDavid Hutchinson
Feb 9, 2018·Nature Reviews. Rheumatology·Hans Ulrich SchererRene E M Toes
Mar 20, 2014·Current Rheumatology Reports·Kevin D Deane
Jul 28, 2012·Tissue Antigens·A Ruyssen-WitrandM Thomsen
Mar 23, 2021·Autoimmunity Reviews·Nora PetrovskáMária Filková

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