Production of biobutanol from cellulose hydrolysate by the Escherichia coli co-culture system

FEMS Microbiology Letters
Mukesh SainiYun-Peng Chao

Abstract

The commercialization of the n-butanol bioprocess is largely dependent on the price of feedstocks. Renewable cellulose appears to be an appealing feedstock. The microbial production of n-butanol still remains challenging because of the limited availability of intracellular NADH. To address this issue, an Escherichia coli strain carrying the clostridial CoA-dependent pathway was supplied with heterologous formate dehydrogenase. With the cellulose hydrolysate of rice straw, this single strain produced cellulosic biobutanol with a production yield at 35% of the theoretical and a productivity of 0.093 g L(-1) h(-1). In an alternative method, the production involved a co-culture system consisting of two separate strains provided with the full CoA-dependent pathway. This system achieved a production yield and productivity reaching 62.8% of the theoretical and 0.163 g L(-1) h(-1), respectively. The result indicates that the E. coli co-culture system is technically promising for the production of cellulosic biobutanol.

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Citations

Jun 1, 2016·Metabolic Engineering·Haoran Zhang, Xiaonan Wang
May 21, 2016·FEMS Microbiology Letters·Michael Sauer
May 8, 2018·Applied Microbiology and Biotechnology·Yujia JiangFengxue Xin
Jun 3, 2021·ACS Synthetic Biology·Alina BurmeisterAlexander Grünberger

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