Production of C5a by ASP, a serine protease released from Aeromonas sobria

The Journal of Immunology : Official Journal of the American Association of Immunologists
Hidetoshi NittaHideo Baba

Abstract

Aeromonas sobria causes pus and edema at sites of infection. However, the mechanisms underlying these effects have not been elucidated. C5a, the amino-terminal fragment of the complement 5th component (C5), mimics these events. To investigate the involvement of C5a in the pathophysiology of A. sobria infection, we examined release of C5a from human C5 by a serine protease (ASP), a putative virulence factor secreted by this bacterium. C5 incubated with enzymatically active ASP induced neutrophil migration in a dose-dependent manner from an ASP concentration of 3 nM and in an incubation time-dependent manner in as little as 7 min, with neutrophil accumulation in guinea pigs at intradermal injection sites and neutrophil superoxide release. These effects on neutrophils were inhibited by a C5a-receptor antagonist. The ASP incubation mixture with C5 but not C3 elicited vascular leakage in a dose- and incubation time-dependent manner, which was inhibited by a histamine H(1)-receptor antagonist. Together with these C5a-like activities, ASP cleaved C5 to release only one C5a Ag, the m.w. of which was similar to that of C5a. Immunoblotting using an anti-C5a Ab revealed generation of a C5a-like fragment from human plasma incubated with AS...Continue Reading

References

Mar 1, 1990·The Journal of Trauma·J D Semel, G Trenholme
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M H de Bruijn, G H Fey
Jan 1, 1986·Annual Review of Immunology·H J Müller-Eberhard
Mar 1, 1987·The Journal of Infectious Diseases·J M Janda, R Brenden
Apr 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·K UdakaH Z Movat
Apr 1, 1981·Journal of Clinical Microbiology·O P DailyC R Lissner
Jan 1, 1984·Journal of Clinical Microbiology·J M JandaE J Bottone
Jan 1, 1981·Annual Review of Biochemistry·K B Reid, R R Porter
Jan 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·W L Gold, I E Salit
Mar 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J M JandaR A Brenden
Aug 1, 1997·The Journal of Allergy and Clinical Immunology·K MaruoH Maeda
Aug 26, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J M Janda, S L Abbott
Aug 19, 1999·The New England Journal of Medicine·M Levi, H Ten Cate
Jan 19, 2002·The Journal of Allergy and Clinical Immunology·Yoko Kikuchi, Allen P Kaplan
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Markus S Huber-LangPeter A Ward
Jul 5, 2005·Critical Care Clinics·William C Aird
Feb 21, 2006·FEMS Microbiology Letters·Hidetomo KobayashiKeinosuke Okamoto
Dec 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takahisa ImamuraKeinosuke Okamoto
Jun 30, 2007·The American Journal of Pathology·Bai-Sui FengPing-Chang Yang
Dec 11, 2007·FEBS Letters·Hidetoshi NittaTakahisa Imamura

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Citations

Aug 6, 2009·The Journal of Biological Chemistry·Hidetomo KobayashiHideaki Tsuge
Mar 19, 2014·PloS One·Eizo TakahashiKeinosuke Okamoto
Dec 4, 2009·Microbiology and Immunology·Hidetomo KobayashiHiroyasu Yamanaka
Jul 23, 2014·Oncology Reports·Hidetoshi NittaTakahisa Imamura
Sep 5, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veronica SchmitzJulio Scharfstein
Mar 19, 2015·The Journal of Biological Chemistry·Hidetomo KobayashiHideaki Tsuge
Jul 19, 2011·Immunology Letters·Fleur BossiFrancesco Tedesco

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