Production of caffeoylmalic acid from glucose in engineered Escherichia coli

Biotechnology Letters
Tianzhen LiTao Liu

Abstract

To achieve biosynthesis of caffeoylmalic acid from glucose in engineered Escherichia coli. We constructed the biosynthetic pathway of caffeoylmalic acid in E. coli by co-expression of heterologous genes RgTAL, HpaBC, At4CL2 and HCT2. To enhance the production of caffeoylmalic acid, we optimized the tyrosine metabolic pathway of E. coli to increase the supply of the substrate caffeic acid. Consequently, an E. coli-E. coli co-culture system was used for the efficient production of caffeoylmalic acid. The final titer of caffeoylmalic acid reached 570.1 mg/L. Microbial production of caffeoylmalic acid using glucose has application potential. In addition, microbial co-culture is an efficient tool for producing caffeic acid esters.

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Citations

Nov 6, 2018·Journal of Industrial Microbiology & Biotechnology·Jillian M HagelPeter J Facchini
Dec 22, 2019·Biotechnology Advances·Xiujuan QianKatrin Ochsenreither
Jun 22, 2021·Critical Reviews in Biotechnology·Linlin WangXue Qiao

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Methods Mentioned

BETA
PCR
NMR

Software Mentioned

ClonExpress

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