PMID: 3770776Jan 1, 1986Paper

Production of Chinese hamster monoclonal antibody to a human cell-surface antigen using a hamster-human somatic cell hybrid as antigen

Hybridoma
D K Palmer, C Jones

Abstract

Hybridomas producing monoclonal antibody (MAb) of predefined specificity were isolated from a Chinese hamster that had received injections of a Chinese hamster-human somatic cell hybrid. A standard method for the production of murine hybridomas was used to produce Chinese spleen lymphocyte X murine plasmacytoma hybridomas, and they were screened for complement-mediated cytotoxicity against a panel of Chinese hamster-human somatic cell hybrids and agglutination of human erythrocytes. Two hybridomas were established in tissue culture following limiting dilution cloning, and their reactivity with a panel of Chinese hamster-human somatic cell hybrids indicates that they are specific for the previously identified human cell-surface antigen a1. The Chinese hamster appears to respond preferentially to human antigens of Chinese hamster-human somatic cell hybrids, and it will serve as a useful tool for the production of MAb specific for human cell-surface antigens expressed in the many well-characterized Chinese hamster-human somatic cell hybrids available.

References

Jul 1, 1973·The Journal of Experimental Medicine·P WuthierT T Puck
Jul 1, 1984·Somatic Cell and Molecular Genetics·C JonesA Tunnacliffe
Jan 1, 1982·Annual Review of Genetics·T T Puck, F T Kao
Jan 1, 1980·Cytogenetics and Cell Genetics·C JonesR T Taylor
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·R G HAM

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