PMID: 16349178Feb 1, 1994

Production of Chitinases and beta-1,3-Glucanases by Stachybotrys elegans, a Mycoparasite of Rhizoctonia solani

Applied and Environmental Microbiology
R J TweddellPierre M Charest

Abstract

The in vitro production of chitinases and beta-1,3-glucanases by Stachybotrys elegans, a mycoparasite of Rhizoctonia solani, was examined under various culture conditions, such as carbon and nitrogen sources, pH, and incubation period. Production of both enzymes was influenced by the carbon source incorporated into the medium and was stimulated by acidic pH and NaNO(3). The activity of both enzymes was very low in culture filtrates from cells grown on glucose and sucrose compared with that detected on chitin (for chitinases) and cell wall fragments (for beta-1,3-glucanases). Protein electrophoresis revealed that, depending on the carbon source used, different isoforms of chitinases and beta-1,3-glucanases were detected. S. elegans culture filtrates, possessing beta-1,3-glucanase and chitinase activities, were capable of degrading R. solani mycelium.

Citations

Oct 24, 2008·Phytopathology·P P SinghY R Chung
Feb 23, 2007·FEMS Microbiology Reviews·Kirstee MartinRobert J Seviour
Apr 23, 2013·Biotechnology Progress·S B Chavan, M V Deshpande
Jan 12, 2007·Canadian Journal of Microbiology·D C MorissetteSuha Jabaji-Hare
Feb 19, 2016·Journal of Basic Microbiology·Chatsuda SakdapetsiriVichien Kitpreechavanich
Jan 6, 2006·Archives of Microbiology·Joanna MuchaAntoni Werner