Production of Cinnamic and p-Hydroxycinnamic Acids in Engineered Microbes

Frontiers in Bioengineering and Biotechnology
Alejandra Vargas-Tah, Guillermo Gosset

Abstract

The aromatic compounds cinnamic and p-hydroxycinnamic acids (pHCAs) are phenylpropanoids having applications as precursors for the synthesis of thermoplastics, flavoring, cosmetic, and health products. These two aromatic acids can be obtained by chemical synthesis or extraction from plant tissues. However, both manufacturing processes have shortcomings, such as the generation of toxic subproducts or a low concentration in plant material. Alternative production methods are being developed to enable the biotechnological production of cinnamic and (pHCAs) by genetically engineering various microbial hosts, including Escherichia coli, Saccharomyces cerevisiae, Pseudomonas putida, and Streptomyces lividans. The natural capacity to synthesize these aromatic acids is not existent in these microbial species. Therefore, genetic modification have been performed that include the heterologous expression of genes encoding phenylalanine ammonia-lyase and tyrosine ammonia-lyase activities, which catalyze the conversion of l-phenylalanine (l-Phe) and l-tyrosine (l-Tyr) to cinnamic acid and (pHCA), respectively. Additional host modifications include the metabolic engineering to increase carbon flow from central metabolism to the l-Phe or l-Tyr ...Continue Reading

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Citations

May 27, 2016·Applied Microbiology and Biotechnology·Shunsuke MasuoNaoki Takaya
Jul 16, 2016·Microbiology·Carlos Molina-SantiagoEstrella Duque
Mar 30, 2017·Applied Microbiology and Biotechnology·Manuela GottardiEckhard Boles
Jun 6, 2018·International Journal of Cosmetic Science·A Gunia-KrzyżakE Pękala
Sep 23, 2018·Applied and Environmental Microbiology·Ashley M FrankAlison Buchan
Dec 7, 2019·Preparative Biochemistry & Biotechnology·Priya UpadhyayArvind Lali
Oct 16, 2019·Frontiers in Bioengineering and Biotechnology·Ronnie J M LubbersRonald P de Vries
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Joana L RodriguesLígia R Rodrigues
Jan 31, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Safa OufensouGiovanna Delogu
Feb 23, 2021·Frontiers in Bioengineering and Biotechnology·Feiyan XueLanqing Ma
Oct 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vladimir I MuronetzMatej Sova

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Methods Mentioned

BETA
deamination
genetic modifications
phosphotransferase
genetic modification
chemical treatment

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