Production of cloned miniature pigs by enucleation using the spindle view system

Reproduction in Domestic Animals = Zuchthygiene
Y LiX Chen

Abstract

Porcine somatic cell nuclear transfer (NT) has been successfully performed, but its efficiency remains quite low. In this study, we improvised on the enucleation method to enhance the development of NT embryos. Initially, an experiment was performed to determine the location relationship between the metaphase plate and the first polar body, where the results showed that the metaphase plate may frequently be displaced during the varying period of maturation process. When the metaphase plates were removed using the 'blind' enucleation method, the enucleation rate was affected by the maturation time; however, when the spindle view system was used, an enucleation rate of 100% was achieved. In the next experiment, these two methods were used to construct embryos: the fusion efficiency was significantly higher (p < 0.01) in the spindle view system group and the development rates of the reconstructed embryos were significantly higher in the spindle view system group compared with the 'blind' enucleation group (p < 0.01). An average of 174 (141-210) cloned embryos from the spindle view system group were transferred into five surrogate pigs and one piglet was delivered at 114 days after embryo transfer by caesarean section. DNA analysis...Continue Reading

References

Sep 1, 1989·Biology of Reproduction·R S PratherN L First
May 20, 1999·Nature Biotechnology·A BaguisiY Echelard
Aug 19, 2000·Science·A OnishiA C Perry
Sep 19, 2000·Nature·I A PolejaevaK H Campbell
Oct 4, 2000·Nature Biotechnology·J BetthauserM Bishop
Feb 23, 2002·Nature·T ShinM Westhusin
Mar 1, 2002·Biology of Reproduction·Jie ZhuIan Wilmut
Apr 23, 2002·Biology of Reproduction·Andrew C BoquestMark B Nottle
May 30, 2003·Biology of Reproduction·Jang-Won LeeXiangzhong Yang
Aug 9, 2003·Nature·Cesare GalliGiovanna Lazzari
Apr 16, 2005·Fertility and Sterility·Paula A A S NavarroDavid L Keefe
Aug 5, 2005·Nature·Byeong Chun LeeWoo Suk Hwang
May 5, 2006·Biology of Reproduction·Rongfeng LiRandall S Prather
Jun 1, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Shangang LiHui Z Sheng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.