Production of cysteinyl-dopamine during intravenous dopamine therapy

Kidney International
J L MagnayA D Baines

Abstract

Oxidized dopamine rapidly forms thiol-conjugates with --SH groups on cysteine, glutathione, and proteins. We used cysteinyl-dopamine production as an index of thioester production during intravenous dopamine treatment of critically ill patients. Cysteinyl-dopamine and catecholamines were measured by high-performance liquid chromatography with electrochemical detection. The production of cysteinyl-dopamine by purified human neutrophils was measured using dopamine (1 micromol/L) and cysteine (1 mmol/L) concentrations similar to those found during dopamine treatment. To examine the impact of endotoxic shock on cysteinyl-dopamine production, anesthetized rats were given dopamine (12 to 15 microg/kg/min intravenously) with or without endotoxin (50 mg/kg intravenously). In vitro, neutrophils converted 26% of dopamine to cysteinyl-dopamine (30 min at 37 degrees C). Activating neutrophils with zymogen increased dopamine consumption from 26 to 68%, but only 36% appeared as cysteinyl-dopamine. The remainder may have been oxidized to other cysteinyl derivatives. Endotoxin increased cysteinyl-dopamine in rat plasma from 2.5 nmol/L (range <0.2 to 11) to 9.7 nmol/L (range <0.3 to 31, P = 0.1). After four hours, with or without endotoxin, cys...Continue Reading

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Citations

Mar 9, 2007·Biomedical Chromatography : BMC·Renata SilvaFernando Remião
Oct 30, 2016·Neuroscience and Biobehavioral Reviews·Viktor S KokhanAndrey S Shtemberg
Nov 13, 2015·Chemistry : a European Journal·Marie KrogsgaardHenrik Birkedal
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Sep 13, 2017·Clinical and Translational Science·David S GoldsteinYehonatan Sharabi
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Apr 26, 2019·High-throughput·Clara Gonçalves-DiasSofia A Pereira

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