Production of cytokeratin 19 fragment by human squamous lung cancer cell lines

American Journal of Respiratory Cell and Molecular Biology
H SatohH Kamma

Abstract

Cytokeratins form part of the cytoskeleton of both normal and malignant epithelium. A novel tumor marker measuring cytokeratin 19 (CK-19) fragment has been introduced and proven to be suitable for monitoring therapy and following cases of non-small cell lung cancer, squamous cell lung cancer in particular. However, whether the serum level of CK-19 fragment reflects the number of proliferating tumor mass remains unknown. We studied the CK-19 fragment produced by two human squamous cell lung cancer cell lines. In Western blotting analysis, culture supernatants of both cell lines displayed bands of 37 and 40 kDa, which represented the CK-19 fragment and the intact CK-19, respectively. Gel filtration demonstrated that a part of soluble CK-19 was released as a large complex form in culture supernatants. The level of CK-19 fragment in culture supernatants increased during the exponential growth phase. CK-19 level decreased by an addition of a cytotoxic agent to non-significant level though the transient release of CK-19 fragment occurred during the first 2 days. After all, soluble CK-19 fragments were detected in culture supernatants of human lung cancer cell lines and its level reflected proliferating cancer cells though it was not ...Continue Reading

Citations

Mar 17, 2001·International Journal of Cancer. Journal International Du Cancer·K DohmotoJ Takahara
Sep 29, 2000·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·K DohmotoJ Takahara
Nov 21, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·J FujitaT Ishida
Dec 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Ken-Ichi OonakaharaMitsuhiro Osame
Jun 29, 2013·Chinese Medicine·Hailian ShiJianqun Xie
Feb 25, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Takamoto SaijoAtsushi Ochiai
Feb 23, 2013·American Journal of Clinical Pathology·Arthur ChoByoung Wook Choi
Jun 11, 1999·International Journal of Cancer. Journal International Du Cancer·Y UedaJ Takahara

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