Production of D-lactic acid by Corynebacterium glutamicum under oxygen deprivation

Applied Microbiology and Biotechnology
Shohei OkinoHideaki Yukawa

Abstract

In mineral salts medium under oxygen deprivation, Corynebacterium glutamicum exhibits high productivity of L-lactic acid accompanied with succinic and acetic acids. In taking advantage of this elevated productivity, C. glutamicum was genetically modified to produce D-lactic acid. The modification involved expression of fermentative D-lactate dehydrogenase (D-LDH)-encoding genes from Escherichia coli and Lactobacillus delbrueckii in L-lactate dehydrogenase (L-LDH)-encoding ldhA-null C. glutamicum mutants to yield strains C. glutamicum DeltaldhA/pCRB201 and C. glutamicum DeltaldhA/pCRB204, respectively. The productivity of C. glutamicum DeltaldhA/pCRB204 was fivefold higher than that of C. glutamicum DeltaldhA/pCRB201. By using C. glutamicum DeltaldhA/pCRB204 cells packed to a high density in mineral salts medium, up to 1,336 mM (120 g l(-1)) of D-lactic acid of greater than 99.9% optical purity was produced within 30 h.

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Citations

Oct 20, 2009·Applied Microbiology and Biotechnology·Toru JojimaHideaki Yukawa
Jun 17, 2010·Applied Microbiology and Biotechnology·Yoshiki TsuchidaHideaki Yukawa
Dec 18, 2010·Applied Microbiology and Biotechnology·Yukihiro TashiroKenji Sonomoto
Feb 18, 2011·Applied Microbiology and Biotechnology·Shogo YamamotoHideaki Yukawa
Nov 19, 2011·Applied Microbiology and Biotechnology·Vipin GopinathK Madhavan Nampoothiri
Dec 1, 2011·Applied Microbiology and Biotechnology·Yuyang SongSeiichi Taguchi
Jan 10, 2012·Applied Microbiology and Biotechnology·Stefan WieschalkaBernhard J Eikmanns
May 25, 2013·Applied Microbiology and Biotechnology·Yukihiro KitadeHideaki Yukawa
Jun 12, 2013·Applied Microbiology and Biotechnology·Noriko AdachiAkihiko Kondo
Oct 31, 2013·Applied Microbiology and Biotechnology·Verena Bückle-VallantBernhard J Eikmanns
Dec 25, 2009·Biotechnology Letters·D S JoshiD V Gokhale
Nov 12, 2010·Applied Biochemistry and Biotechnology·Huimin LiuGuanjun Chen
Apr 21, 2009·Applied and Environmental Microbiology·Haruhiko TeramotoHideaki Yukawa
Jul 1, 2008·Applied and Environmental Microbiology·Haruhiko TeramotoHideaki Yukawa
Jul 6, 2010·Applied and Environmental Microbiology·Haruhiko TeramotoHideaki Yukawa
Oct 12, 2010·Applied and Environmental Microbiology·Felix S KrauseBernhard J Eikmanns
Apr 7, 2009·Applied and Environmental Microbiology·Hideo KawaguchiHideaki Yukawa
Mar 29, 2011·Applied and Environmental Microbiology·Bastian BlombachBernhard J Eikmanns
Apr 20, 2010·Applied and Environmental Microbiology·Akos T KovácsRichard van Kranenburg
Jan 25, 2011·Journal of Bacteriology·Norma StäblerLothar Eggeling
Dec 20, 2011·Journal of Bacteriology·Tanja LasloBernhard J Eikmanns
Apr 30, 2013·Biotechnology Advances·Mohamed Ali Abdel-RahmanKenji Sonomoto
May 27, 2014·Journal of Industrial Microbiology & Biotechnology·Chen WangPingkai Ouyang
Aug 20, 2015·Critical Reviews in Biotechnology·Veeresh Juturu, Jin Chuan Wu
Jan 23, 2016·Microbial Cell Factories·Yota TsugeAkihiko Kondo
Oct 2, 2015·Applied Biochemistry and Biotechnology·Qingxin LiJin Chuan Wu

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