Production of DagA and ethanol by sequential utilization of sugars in a mixed-sugar medium simulating microalgal hydrolysate

Bioresource Technology
Juyi ParkYong Keun Chang

Abstract

A novel two-step fermentation process using a mixed-sugar medium mimicking microalgal hydrolysate has been proposed to avoid glucose repression and thus to maximize substrate utilization efficiency. When DagA, a β-agarase was produced in one step in the mixed-sugar medium by using a recombinant Streptomyces lividans, glucose was found to have negative effects on the consumption of the other sugars and DagA biosynthesis causing low substrate utilization efficiency and low DagA productivity. To overcome such difficulties, a new strategy of sequential substrate utilization was developed. In the first step, glucose was consumed by Saccharomyces cerevisiae together with galactose and mannose producing ethanol, after which DagA was produced from the remaining sugars of xylose, rhamnose and ribose. Fucose was not consumed. By adopting this two-step process, the overall substrate utilization efficiency was increased approximately 3-fold with a nearly 2-fold improvement of DagA production, let alone the additional benefit of ethanol production.

References

Dec 1, 1980·Pharmacology, Biochemistry, and Behavior·W WetzelH Matthies
Jan 1, 1997·Bioscience, Biotechnology, and Biochemistry·R KobayashiS Usami
Jul 31, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·I Fodil-BourahlaL Robert
Feb 5, 2005·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·C RobertL Robert
Aug 8, 2006·Biotechnology Journal·Assunta GiordanoAntonio Trincone
Jul 29, 2010·Bioresource Technology·Rojan P JohnAshok Pandey
Nov 3, 2012·Bioresource Technology·Shih-Hsin HoJo-Shu Chang
May 18, 2013·Applied Microbiology and Biotechnology·Jae Sun LeeYong Keun Chang
Mar 13, 2014·Bioprocess and Biosystems Engineering·Jae Hyung KimYong Keun Chang

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Citations

Jan 1, 2019·Biotechnology and Bioengineering·Jing-Jing LiuYong-Su Jin
Oct 10, 2018·Biotechnology Progress·Gyeongho SeonYong Keun Chang
Oct 9, 2020·Scientific Reports·Gyeongho SeonYong Keun Chang

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