Production of diacetyl by metabolically engineered Enterobacter cloacae

Scientific Reports
Lijie ZhangCuiqing Ma

Abstract

Diacetyl, a high value product that can be extensively used as a food ingredient, could be produced from the non-enzymatic oxidative decarboxylation of α-acetolactate during 2,3-butanediol fermentation. In this study, the 2,3-butanediol biosynthetic pathway in Enterobacter cloacae subsp. dissolvens strain SDM, a good candidate for microbial 2,3-butanediol production, was reconstructed for diacetyl production. To enhance the accumulation of the precursor of diacetyl, the α-acetolactate decarboxylase encoding gene (budA) was knocked out in strain SDM. Subsequently, the two diacetyl reductases DR-I (gdh) and DR-II (budC) encoding genes were inactivated in strain SDM individually or in combination to decrease the reduction of diacetyl. Although the engineered strain E. cloacae SDM (ΔbudAΔbudC) was found to have a good ability for diacetyl production, more α-acetolactate than diacetyl was produced simultaneously. In order to enhance the nonenzymatic oxidative decarboxylation of α-acetolactate to diacetyl, 20 mM Fe(3+) was added to the fermentation broth at the optimal time. In the end, by using the metabolically engineered strain E. cloacae SDM (ΔbudAΔbudC), diacetyl at a concentration of 1.45 g/L was obtained with a high productivi...Continue Reading

References

Aug 31, 2000·Applied and Environmental Microbiology·J HugenholtzP Hols
Nov 9, 2007·Trends in Biotechnology·Ping XuCuiqing Ma
Oct 25, 2008·Applied Microbiology and Biotechnology·Cuiqing MaPing Xu
May 16, 2009·Biotechnology Advances·E Celińska, W Grajek
Jan 29, 2011·Biotechnology Advances·Xiao-Jun JiPing-Kai Ouyang
May 15, 2013·Applied Microbiology and Biotechnology·Liaoyuan ZhangYaling Shen
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Feb 19, 2014·Applied Microbiology and Biotechnology·Chuan ChenJian Hao
Jul 2, 2014·Journal of Industrial Microbiology & Biotechnology·Liaoyuan ZhangYaling Shen

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Citations

Oct 2, 2015·Current Opinion in Biotechnology·Austin L CarrollShota Atsumi
Jun 27, 2015·Applied Biochemistry and Biotechnology·Jeongmo YangJinwon Lee
Nov 20, 2016·Scientific Reports·Vijayalakshmi KandasamyPeter Ruhdal Jensen
Mar 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuanzhi HeJun Yuan
Jan 21, 2021·Biotechnology for Biofuels·Hsiang-Yen SuKe-Ke Cheng

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Methods Mentioned

BETA
gene knock-out
PCR

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