PMID: 3756143Jul 29, 1986Paper

Production of dihydrothymidine stereoisomers in DNA by gamma-irradiation

Biochemistry
E A FurlongW D Henner

Abstract

5,6-Dihydrothymidine (dDHT) is a derivative thymidine formed during gamma-irradiation. This paper demonstrates the conditions under which dDHT is formed in solutions of DNA and that dDHT is produced in the DNA of HeLa cells during gamma-irradiation. The product of dDHT by gamma-irradiation of either thymidine or DNA has been quantitated by a sensitive and specific high-pressure liquid chromatography method. dDHT is a major product of the anoxic irradiation of thymidine (G value 0.5) but is produced in substantially smaller amounts in DNA irradiated under the same conditions (G value 0.026). The presence of oxygen reduces the yield of dDHT by at least 25-fold for both irradiation substrates. In HeLa cells, 60Co irradiation under anoxia produces (6.2 +/- 0.2) X 10(-8) mol of the R isomer of dDHT per mole of cell deoxynucleotide per gray (G value 0.11). gamma-Irradiation of thymidine produces equal quantities of the R and S stereoisomers of dDHT. Irradiation of DNA produces significantly more (69%) (R)- than (S)-dDHT. DNA isolated from cultured human cells following gamma-irradiation also contains more of the R than the S form of dDHT. The conformation of double-stranded DNA favors a stereospecific production of the R isomer. Amon...Continue Reading

Citations

Oct 23, 1987·Journal of Immunological Methods·J A VilpoL M Vilpo
Jan 1, 1991·Free Radical Biology & Medicine·M Dizdaroglu
Jun 1, 1995·Free Radical Biology & Medicine·A P Breen, J A Murphy
Sep 1, 1992·Mutation Research·M Dizdaroglu
Apr 1, 1991·Journal of Biomolecular Structure & Dynamics·Y VaishnavL S Kan
Feb 25, 2009·Journal of Biomolecular Structure & Dynamics·N A TimofeyevaO S Fedorova
May 17, 2011·Biochemistry. Biokhimii︠a︡·N A TimofeyevaO S Fedorova
Jan 6, 2004·Nucleic Acids Research·Laurent GrosMurat K Saparbaev
Jun 30, 2004·International Journal of Radiation Biology·J B DawidzikH C Box
Aug 19, 2008·Mutation Research·Teresa Roldán-Arjona, Rafael R Ariza
Jul 19, 2015·Ultrasonics Sonochemistry·Jisha ChandranC T Aravindakumar
Jan 27, 2012·Free Radical Research·Miral Dizdaroglu, Pawel Jaruga
Jan 24, 2002·Nature·Alexander A Ischenko, Murat K Saparbaev
Oct 29, 2002·The Journal of Biological Chemistry·Rhoderick H Elder, Grigory L Dianov
Feb 29, 2020·The Biochemical Journal·Noe Baruch-TorresLuis G Brieba
Aug 1, 1988·International Journal of Radiation Biology·G W TeeborJ Cadet
Feb 1, 1992·International Journal of Radiation Biology·M Dizdaroglu
Apr 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R Téoule
Jan 29, 2008·Radiation Research·Bulent AydoganDavid T Marshall
Jan 1, 1992·Free Radical Research Communications·Z NackerdienM Dizdaroglu
Nov 1, 1988·International Journal of Radiation Biology·M Liuzzi, M Talpaert-Borlé
Sep 1, 1990·International Journal of Radiation Biology·A F FuciarelliM Dizdaroglu
Aug 20, 2019·Bioorganic & Medicinal Chemistry·Toshinori Suzuki, Yuki Kishida
Feb 1, 2013·EcoSal Plus·Sophie CouvéMurat Saparbaev
Sep 28, 2017·Journal of Agricultural and Food Chemistry·Naoki FukuiHirotaka Obana

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