Production of diosgenin from Dioscorea zingiberensis tubers through enzymatic saccharification and microbial transformation

Applied Microbiology and Biotechnology
Yuling ZhuHui Li

Abstract

In order to develop a clean and effective approach for producing the valuable drug diosgenin from Dioscorea zingiberensis tubers, two successive processes, enzymatic saccharification and microbial transformation, were used. With enzymatic saccharification, 98.0% of starch was excluded from the raw herb, releasing saponins from the network structure of starch. Subsequently, the treated tubers were fermented with Trichoderma reesei under optimal conditions for 156 h. During microbial transformation, glycosidic bonds, which link beta-D-glucose or alpha-L-rhamnose with aglycone at the C-3 position in saponins, were broken down effectively to give a diosgenin yield of 90.6+/-2.45%, 42.4% higher than that obtained from bioconversion of raw tubers directly. Scaled up fermentation was conducted in a 5.0-l bioreactor and gave a diosgenin yield of 91.2+/-3.21%. This is the first report on the preparation of diosgenin from herbs through microbial transformation as well as utilizing other available components in the raw material, providing an environmentally friendly alternative to diosgenin production.

References

Oct 1, 1996·Planta medica·H HasegawaM Uchiyama
Mar 3, 2007·Applied Microbiology and Biotechnology·H WangZ-L Xiu
Oct 24, 2007·Applied Microbiology and Biotechnology·Feng-Qing WangDong-Zhi Wei
Nov 28, 2007·Applied Microbiology and Biotechnology·Guang-Tong ChenDe-An Guo
Feb 24, 2009·Molecular and Cellular Biochemistry·K S JayachandranG V Rajamanickam
Mar 21, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·J BertrandS Battu
May 12, 2009·Steroids·Yayoi TadaShinichi Watanabe

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Citations

Jul 19, 2013·World Journal of Microbiology & Biotechnology·Yuling ZhuJinren Ni
Sep 6, 2013·Journal of Industrial Microbiology & Biotechnology·Nguyen Huy Thuan, Jae Kyung Sohng
Sep 15, 2014·Journal of Ethnopharmacology·Hwayong ParkJin Yeul Ma
Jun 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi HeShuiyuan Cheng
May 21, 2020·The American Journal of Chinese Medicine·Lin LiChunjie Wu
May 12, 2018·Planta·Swati UpadhyayRakesh Kumar Shukla
Mar 25, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Samrin ShaikhVinay Kumar
Apr 11, 2021·Chemistry & Biodiversity·Poonam RawatSharad Srivastava

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