Production of eicosanoids derived from 20:4n-6 and 20:5n-3 in primary cultures of turbot (Scophthalmus maximus) brain astrocytes in response to platelet activating factor, substance P and interleukin-1 beta

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
D R TocherJ R Sargent

Abstract

Primary cultures of turbot (Scophthalmus maximus) brain astroglial cells established in medium containing fetal bovine serum contain increased proportions of 18:1(n-9), total (n-9) and (n-6) polyunsaturated fatty acids (PUFA) and greatly reduced (n-3) PUFA in comparison with turbot brain. Supplementation with a mixture of 5 microM eicosapentaenoic [20:5(n-3)] and 25 microM docosahexaenoic [22:6(n-3)] acids for 4 days significantly increased the percentages of these acids in total cellular lipid of turbot astrocytes and restored the (n-3) PUFA composition of the cells to that found in turbot brain. The production of prostaglandins (PG) E and F of the 2- and 3-series and leukotrienes (LT) C4 and C5 in response to various agonists was determined in PUFA-supplemented astrocytes. Calcium ionophore A23187, platelet activating factor and substance P stimulated the production of both PGF and PGE. Interleukin-1 beta significantly stimulated the production of PGF only. There were differences between the agonists in their effects on the relative levels of 2- and 3-series PGs produced. Only very low amounts of LTC were produced by the turbot astrocytes, with only substance P showing a minor stimulatory effect.

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Citations

Jun 10, 2010·Fish Physiology and Biochemistry·N Y Mohd-YusofD R Tocher
Apr 18, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·José L Soengas, Manuel Aldegunde
Dec 13, 2005·Journal of Neuroscience Methods·Virginia MartínMario Díaz
Jun 14, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wen LiuRichard J Kulmacz
Oct 25, 2011·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Seyed-Mohammadreza Samaee, Alicia Estévez
Dec 28, 2006·Medicinal Research Reviews·Eleni Pontiki, Dimitra Hadjipavlou-Litina
Nov 26, 2020·Animals : an Open Access Journal From MDPI·Agata KowalskaRadosław K Kowalski

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