PMID: 8448018Mar 1, 1993Paper

Production of eicosanoids in response to endothelin-1 and identification of specific endothelin-1 binding sites in airway epithelial cells

American Journal of Respiratory Cell and Molecular Biology
T WuJ H Shelhamer

Abstract

The effect of endothelin-1 (ET-1) on arachidonate metabolism in the respiratory epithelium was investigated in primary cultures of feline tracheal epithelial cells. Subconfluent epithelial cell cultures were stimulated by ET-1, and eicosanoid generation was determined by high performance liquid chromatography (HLPC) of 3H-labeled arachidonic acid (AA) metabolites and by radioimmunoassay (RIA) of corresponding nonradiolabeled HPLC elution. The HPLC chromatograms of [3H]AA-prelabeled samples revealed that ET-1 (10(-5) M) augmented the release of prostaglandin (PG) E2, 12-hydroxyeicosatetraenoic acid (HETE), PGF2 alpha, and AA. RIA of corresponding nonradiolabeled HPLC elution demonstrated a significantly increased release of PGE2, PGF2 alpha, and 12-HETE as well as 5-HETE in response to ET-1 stimulation. 5-HETE release from ET-1-stimulated cells was further identified by gas chromatography/mass spectrometry (GC/MS). The stimulating effect of ET-1 on AA metabolism was dose dependent (10(-5) to 10(-7) M) and peaked within 1 h with a progressive decline over the subsequent hours. Using 125I-labeled ET-1 as radioligand, the presence of specific binding sites for ET-1 was demonstrated in cultured feline tracheal epithelial cells. ET-1...Continue Reading

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Citations

Oct 2, 2003·Respiratory Physiology & Neurobiology·Mathieu SimonJohn Tsang
Feb 1, 1994·The Journal of Clinical Investigation·T WuJ H Shelhamer
Sep 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·F H HsiehS J Haque
Jul 25, 2013·Pharmacology & Therapeutics·Delphine GrasArnaud Bourdin
Jan 29, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J Mullol, C Picado
Jul 4, 2003·Canadian Journal of Physiology and Pharmacology·Bruno Battistini
Jul 14, 1999·Life Sciences·R G Goldie, P J Henry

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