Production of elevated levels of dextransucrase by a mutant of Streptococcus mutans.

Infection and Immunity
C F SchachteleS K Harlander

Abstract

A mutant (S19) of Sreptococcus mutans strain 6715 which produces elevated levels of dextransucrase (EC 2.4.1.5) was isolated. Soluble enzyme in culture supernatant solutions from S19 polymerized the glucosyl moiety of sucrose into alcohol-insoluble and water-insoluble glucans at a rate three to six times greater than that of the parent strain. Washed-cell suspensions of S19 also contained increased amounts of cell-associated enzyme. Adherence of S19 to glass in the presence of sucrose occurred at twice the rate of strain 6715. The Km values for sucrose and primer dextran were similar for the mutant and parent enzymes. Mutant S19 should facilitate studies on the mechanism of adherence of S. Mutans and the control of dextransucrase production by this bacterium.

References

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Citations

Aug 1, 1977·Journal of Dental Research·T J MontvilleA J Sinskey
Oct 1, 1980·Journal of Dental Research·J J BozzolaI L Shechmeister
Feb 1, 1977·Infection and Immunity·S M MichalekD Devenyns
Jun 1, 1984·Applied and Environmental Microbiology·T W Feary, J A Mayo
Jan 1, 1978·Journal of Bacteriology·C L WittenbergerL N Lee
Jan 1, 1980·Infection and Immunity·T IkedaT Shiota
Dec 1, 1991·Journal of Bacteriology·P Lawman, A S Bleiweis
Feb 1, 1978·Infection and Immunity·S K Harlander, C F Schachtele
Jun 1, 1980·Microbiological Reviews·S Hamada, H D Slade

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