Abstract
Plant cell and organ cultures of Helianthella quinquenervis, a medicinal plant whose roots are used by the Tarahumara Indians of Chihuahua, Mexico, to relieve several ailments, were established to identify and quantify some chromenes with biological activity, such as encecalin, and to evaluate their potential for biotechnological production. Gas chromatography-mass spectrometry (GC-MS) analysis corroborated the presence of quantifiable amounts of encecalin in H. quinquenervis cell cultures (callus and cell suspensions). In addition, hairy roots were obtained through three transformation protocols (prick, 45-s sonication and co-culture), using wild type Agrobacterium rhizogenes A4. After three months, cocultivation achieved the highest percentage of transformation (66%), and a comparable production (FW) of encecalin (110 μg/g) than the sonication assay (120 μg/g), both giving far higher yields than the prick assay (19 μg/g). Stable integration of rolC and aux1 genes in the transformed roots was confirmed by polymerase chain reaction (PCR). Hairy roots from cocultivation (six months-old) accumulated as much as 1086 μg/g (FW) of encecalin, over three times higher than the cell suspension cultures. The production of encecalin varie...Continue Reading
References
May 15, 1989·Gene·T AoyamaA Oka
Jun 7, 1985·Science·M F BalandrinW H Bollinger
Mar 1, 1996·Journal of Natural Products·P CastañedaR Bye
Oct 6, 2000·Phytochemistry·I SaadM Jiménez-Estrada
Nov 15, 2000·Journal of Natural Products·I S RojasR Mata
Oct 11, 2003·Biotechnology Advances·S R Rao, G A Ravishankar
Apr 18, 2006·Current Opinion in Plant Biology·Stéphanie GuillonPascal Gantet
May 17, 2006·Trends in Ecology & Evolution·Karina Boege, Robert J Marquis
Jun 30, 2006·Plant Physiology and Biochemistry : PPB·Nam-Soo JwaRandeep Rakwal
Apr 1, 2008·Current Opinion in Biotechnology·Shuiqin Wu, Joe Chappell
Jan 16, 2010·Functional & Integrative Genomics·Tabasum KawoosaSanjay Kumar
Dec 15, 2010·Journal of Ethnopharmacology·Angel Josabad Alonso-CastroAlejandro Garcia-Carranca
Dec 25, 2010·Plant Cell Reports·Milen I GeorgievAnnemarie Lippert
May 29, 2012·Biotechnology Letters·Agata Wilczańska-BarskaMirosława Krauze-Baranowska
Mar 1, 1996·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·M T Gutierrez-LugoR Mata
Aug 24, 2013·Journal of Medicinal Chemistry·Dipak HarelBernhard Wünsch
Mar 1, 1996·Journal of Chemical Ecology·A KunzeP Proksch
Jul 1, 1989·Journal of Chemical Ecology·G B Merrill
Jun 26, 2014·Critical Reviews in Biotechnology·Wei YueLu-Ping Qin
Dec 25, 2015·BMB Reports·Marisol Ochoa-VillarrealGary J Loake
Mar 26, 2016·Frontiers in Microbiology·Aye ThweSang Un Park
Jun 3, 2016·BMC Plant Biology·Erum DilshadBushra Mirza
Jul 1, 1998·Plant Cell Reports·E R SantarémJ J Finer
Jul 14, 2019·Applied Biochemistry and Biotechnology·Orapin UdomsinWaraporn Putalun
Mar 21, 2020·Frontiers in Plant Science·Noemi Gutierrez-ValdesFlorian Cardon