PMID: 6987658Jan 1, 1980Paper

Production of erythroid-potentiating activity by a human T-lymphoblast cell line

Proceedings of the National Academy of Sciences of the United States of America
D W GoldeA J Lusis

Abstract

We derived a human T-lymphoblast cell line (Mo) that constitutively elaborates certain lymphokines. The Mo cells produce a colony-stimulating factor necessary for the growth of human granulocyte-monocyte precursors in vitro as well as an erythroid-potentiating activity (EPA) that enhances the proliferation of human erythroid progenitors in vitro. In the presence of serum, the EPA in Mo-conditioned medium stimulated the growth of small and large erythroid colonies almost 2-fold. EPA was also produced in serum-free medium, and, when assayed in serum-free cultures of human erythroid progenitors, it stimulated colony growth about 3-fold. The EPA produced by the Mo cell line did not stimulate normal murine erythroid progenitors (CFU-E) or Friend erythroleukemia cell growth in vitro. EPA was inactivated by protease treatment but was remarkably heat stable, with most of the activity recovered after boiling for 15 min. Preliminary biochemical characterization suggests that EPA is an acidic glycoprotein with molecular weight approximately 45,000. EPA is clearly separable from colony-stimulating factor on the basis of heat stability and gel-filtration chromatography. The present observations provide strong support for the concept that ac...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G R Johnson, D Metcalf
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D W GoldeC H Li
Mar 1, 1978·Annals of Internal Medicine·A SaxonD W Golde
Jan 18, 1979·Nature·M J Cline, D W Golde
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Nov 1, 1972·The Journal of Clinical Investigation·D W Golde, M J Cline

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Citations

May 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·B M ScherS Waxman
May 9, 2006·Cancer Metastasis Reviews·Rosemarie ChircoHyeong-Reh Choi Kim
Jan 1, 1981·International Journal of Immunopharmacology·D G Nathan
Nov 13, 1980·The New England Journal of Medicine·D W GoldeC H Li
Jul 2, 1981·The New England Journal of Medicine·J H FitchenM J Cline
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T Shibuya, T W Mak
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P TambourinB Varet
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·I S ChenD W Golde
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K WelteM A Moore
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L FeldmanN Dainiak
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S R PaulD A Williams
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M D LairmoreJ E Kaplan
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T T Yang, S P Hawkes
Oct 1, 1987·British Journal of Haematology·G Migliaccio, A R Migliaccio
Sep 1, 1981·The Journal of Clinical Investigation·E M MazurE Bruno
Apr 1, 1982·The Journal of Clinical Investigation·D Douer, H P Koeffler
Oct 1, 1982·The Journal of Clinical Investigation·R M MacklisD G Nathan
Feb 1, 1985·The Journal of Clinical Investigation·W VainchenkerJ P Cartron
Aug 1, 1981·Blut·J Neuwirt
Jan 1, 1987·Critical Reviews in Oncology/hematology·G TrinchieriB Perussia
Jan 1, 1985·Critical Reviews in Oncology/hematology·A M Gewirtz, R Hoffman
Jun 1, 1983·Cellular Immunology·H G RemoldD W Golde
Sep 1, 1989·British Journal of Haematology·P H TsaiJ M Lipton
Dec 1, 1981·British Journal of Haematology·D DouerH P Koeffler
May 1, 1986·International Journal of Cell Cloning·J L Spivak
Sep 1, 1986·International Journal of Cell Cloning·E S StrobelP L Greenberg
Nov 1, 1987·International Journal of Cell Cloning·A Kanamaru, H Hara

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