Production of extended-spectrum β-lactamases and the potential indirect pathogenic role of Prevotella isolates from the cystic fibrosis respiratory microbiota

International Journal of Antimicrobial Agents
Laura J SherrardMichael Tunney

Abstract

Extended-spectrum β-lactamase (ESBL) production and the prevalence of the β-lactamase-encoding gene blaTEM were determined in Prevotella isolates (n=50) cultured from the respiratory tract of adults and young people with cystic fibrosis (CF). Time-kill studies were used to investigate the concept of passive antibiotic resistance and to ascertain whether a β-lactamase-positive Prevotella isolate can protect a recognised CF pathogen from the action of ceftazidime in vitro. The results indicated that approximately three-quarters (38/50; 76%) of Prevotella isolates produced ESBLs. Isolates positive for ESBL production had higher minimum inhibitory concentrations (MICs) of β-lactam antibiotics compared with isolates negative for production of ESBLs (P<0.001). The blaTEM gene was detected more frequently in CF Prevotella isolates from paediatric patients compared with isolates from adults (P=0.002), with sequence analysis demonstrating that 21/22 (95%) partial blaTEM genes detected were identical to blaTEM-116. Furthermore, a β-lactamase-positive Prevotella isolate protected Pseudomonas aeruginosa from the antimicrobial effects of ceftazidime (P=0.03). Prevotella isolated from the CF respiratory microbiota produce ESBLs and may influ...Continue Reading

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Citations

Feb 8, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Debby Bogaert, Alex van Belkum
Aug 18, 2018·Expert Review of Respiratory Medicine·Lindsay J Caverly, John J LiPuma
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Jun 28, 2018·The European Respiratory Journal·Marianne S MuhlebachMichael M Tunney
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Mar 25, 2019·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Laura J SherrardMichael M Tunney
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Sep 17, 2021·European Respiratory Review : an Official Journal of the European Respiratory Society·Sara Van den BosscheAurélie Crabbé

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