PMID: 6984190Dec 1, 1982Paper

Production of functional human T-T hybridomas in selection medium lacking aminopterin and thymidine

Proceedings of the National Academy of Sciences of the United States of America
S K FoungE G Engleman

Abstract

The production of hybridomas between immunologically activated T cells and malignant T-cell lines offers a potentially unlimited source of soluble T-cell-derived products. Recently, human T-T hybrids have been described; however, their use has been hampered by slow growth and chromosomal instability due at least in part to the presence of thymidine in the traditional hypoxanthine/aminopterin/thymidine (HAT) selection medium. In this report, we describe the development of a rapidly growing hypoxanthine phosphoribosyltransferase-deficient human T-cell line designated J3R7, the use of azaserine/hypoxanthine (AH) medium as an alternative selection medium to HAT medium, and the production of functional T-T hybrids by using the J3R7 line and the AH selection technique. Hybrids selected in AH medium were 4-fold greater in number and 3-fold faster in growth rate than hybrids grown in HAT medium. No stable clones were obtained from HAT cultures whereas AH-derived hybrids could be readily cloned by the method of limiting dilution. Evidence for hybridization included (i) the presence of approximately twice the number of chromosomes in hybrids than in J3R7 cells; (ii) the presence on hybrid cells of the Leu-3a surface antigen, present on n...Continue Reading

References

Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M OkadaT Kishimoto
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Dec 1, 1981·The Journal of Experimental Medicine·O IrigoyenL Chess

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Citations

Jul 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·N YabeM Takada
May 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·Y MyokenJ D Sato
Feb 28, 1985·Journal of Immunological Methods·N KouttabA L Maizel
Aug 2, 1985·Journal of Immunological Methods·L MayerH G Kunkel
Jun 10, 1986·Journal of Immunological Methods·G GallagherW H Stimson
Mar 1, 1990·Veterinary Immunology and Immunopathology·J O Olobo, S J Black
Mar 1, 1991·Brain Research. Molecular Brain Research·C J CosciaM R Hanley
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T Manser, M L Gefter
Sep 1, 1983·The Journal of Experimental Medicine·J FujimotoR Levy
Sep 15, 1987·International Journal of Cancer. Journal International Du Cancer·A B GriffithsJ Taylor-Papadimitriou
Dec 1, 1986·Scandinavian Journal of Clinical and Laboratory Investigation·V P CollinsA Carlström
Apr 1, 1993·The Journal of Investigative Dermatology·I A KingA I Magee

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