Production of glutaric acid from 5-aminovaleric acid using Escherichia coli whole cell bio-catalyst overexpressing GabTD from Bacillus subtilis

Enzyme and Microbial Technology
Yun-Gi HongYung-Hun Yang

Abstract

Glutaric acid is one of the promising C5 platform compounds in the biochemical industry. It can be produced chemically, through the ring-opening of butyrolactone followed by hydrolysis. Alternatively, glutaric acid can be produced via lysine degradation pathways by microorganisms. In microorganisms, the overexpression of enzymes involved in this pathway from E. coli and C. glutamicum has resulted in high accumulation of 5-aminovaleric acid. However, the conversion from 5-aminovaleric acid to glutaric acid has resulted in a relatively low conversion yield for unknown reasons. In this study, as a solution to improve the production of glutaric acid, we introduced gabTD genes from B. subtilis to E. coli for a whole cell biocatalytic approach. This approach enabled us to determine the effect of co-factors on reaction and to achieve a high conversion yield from 5-aminovaleric acid at the optimized reaction condition. Optimization of whole cell reaction by different plasmids, pH, temperature, substrate concentration, and cofactor concentration achieved full conversion with 100 mM of 5-aminovaleric acid to glutaric acid. Nicotinamide adenine dinucleotide phosphate (NAD(P)+) and α-ketoglutaric acid were found to be critical factors in t...Continue Reading

Citations

Nov 20, 2018·Biotechnology and Bioengineering·Yun-Gi HongYung-Hun Yang
Jan 25, 2021·Bioprocess and Biosystems Engineering·Yeong-Hoon HanYung-Hun Yang
Nov 6, 2020·Journal of Microbiology and Biotechnology·Ye-Lim ParkYung-Hun Yang
Feb 27, 2021·Frontiers in Bioengineering and Biotechnology·Jie ChengJingwen Zhou
Apr 29, 2021·Applied Microbiology and Biotechnology·Jiaping WangLiming Liu

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