Production of GP64-free virus-like particles from baculovirus-infected insect cells

The Journal of General Virology
Lorena C S ChavesGary W Blissard

Abstract

The retroviral Gag protein is frequently used to generate 'virus-like particles' (VLPs) for a variety of applications. Retroviral Gag proteins self-assemble and bud at the plasma membrane to form enveloped VLPs that resemble natural retrovirus virions, but contain no viral genome. The baculovirus expression vector system has been used to express high levels of the retroviral Gag protein to produce VLPs. However, VLP preparations produced from baculovirus-infected insect cells typically contain relatively large concentrations of baculovirus budded virus (BV) particles, which are similar in size and density to VLPs, and thus may be difficult to separate when purifying VLPs. Additionally, these enveloped VLPs may have substantial quantities of the baculovirus-encoded GP64 envelope protein in the VLP envelope. Since VLPs are frequently produced for vaccine development, the presence of the GP64 envelope protein in VLPs, and the presence of Autographa californica multicapsid nucleopolyhedrovirus BVs in VLP preparations, is undesirable. In the current studies, we developed a strategy for reducing BVs and eliminating GP64 in the production of VLPs, by expressing the human immunodeficiency virus type 1 gag gene in the absence of the bac...Continue Reading

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Citations

Mar 19, 2020·Journal of Separation Science·Katrin ReiterAlois Jungbauer
Oct 1, 2020·Molecular Biology Reports·Leonardo Lopes-LuzTatsuya Nagata
Apr 27, 2019·Archives of Virology·Raquel Medeiros VasquesDaniel M P Ardisson-Araújo
Jul 17, 2020·Journal of Biotechnology·Eduard Puente-MassaguerMartí Lecina
Mar 20, 2019·Industrial & Engineering Chemistry Research·Christine M YeeFei Wen
Jul 7, 2021·Molecular Biotechnology·Thaissa Consoni BernardinoSoraia Attie Calil Jorge

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