Production of group C streptococcus phage-associated lysin and the preparation of Streptococcus pyogenes protoplast membranes

Journal of General Microbiology
J WheelerJ D Blainey

Abstract

Optimum conditions of pH, temperature and multiplicity of bacteriophage infection for the production of phage-associated lysin from Group C streptococci were defined. Lysin obtained by the optimum method was used to make protoplast membranes of Group A type 12 streptococci. These membranes consistently contained very low concentrations of rhamnose, indicating minimal cell wall contamination. The development of such a reproducible technique for the preparation of protoplast membranes will permit more meaningful studies on the involvement of these membranes in the pathogenesis of acute post-streptococcal glomerulonephritis.

Citations

May 20, 2003·Journal of Bacteriology·Daniel NelsonVincent A Fischetti
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·D NelsonV A Fischetti
Jul 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Daniel NelsonVincent A Fischetti
Aug 13, 2015·Public Understanding of Science·Maureen Burns
Jul 6, 2000·The Journal of Biological Chemistry·I Bruck, M O'Donnell

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