PMID: 8960401Sep 1, 1996Paper

Production of heparin-binding endothelial mitogens by bovine uterine fibroblastic and epithelial cells

Canadian Journal of Physiology and Pharmacology
M L JohnsonL P Reynolds

Abstract

This study was conducted to determine whether early-passage cultures of bovine endometrial fibroblastic (Fb, n = 7 uteri) and epithelial (Ep, n = 3 uteri) cells produce endothelial mitogens in vitro and to begin characterization of these mitogens. Confluent cultures of Fb and Ep were incubated for 72 h in serum-free media, and the resulting conditioned media (CM) were evaluated for effects on proliferation of bovine aortic endothelial cells. CM from these Fb cultures (n = 8) and Ep cultures (n = 4) stimulated (147 +/- 10% (mean +/- SEM), p < 0.01, and 124 +/- 8%, p < 0.10, respectively) proliferation of endothelial cells compared with control (unconditioned) media. Most of the mitogenic activity of a sample of Fb CM and a sample of Ep CM from one individual uterus bound to heparin-agarose, and each exhibited two major peaks of activity that eluted at 0.9-1.0 and 1.7-1.8 M NaCl; the Fb CM also exhibited an additional heparin-binding peak eluting at 0-0.1 M NaCl. Pooled Fb CM (n = 8 cultures from 7 animals) also contained mitogenic activity for endothelial cells that bound to heparin-agarose, but exhibited three major peaks, eluting at 0.6, 1.1, and 1.8 M NaCl. Pooled Ep CM (n = 4 cultures from 3 animals) showed only one peak of ...Continue Reading

References

May 1, 1990·Endocrine Reviews·C L Clarke, R L Sutherland
Dec 1, 1986·The Journal of Endocrinology·J K Findlay
Jan 1, 1989·Annual Review of Biochemistry·W H Burgess, T Maciag
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D MoscatelliD B Rifkin
Apr 30, 1985·Biochemical and Biophysical Research Communications·D GospodarowiczP Böhlen

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