Production of horsegram (Dolichos biflorus) Bowman-Birk inhibitor by an intein mediated protein purification system

Protein Expression and Purification
Vinod Kumar, L R Gowda


The seeds of the legume horsegram (Dolichos biflorus), a protein rich pulse (bean), contain multiple forms of Bowman-Birk inhibitors (protease inhibitors). The major inhibitor HGI-III contains seven interweaving disulfides and is extremely stable to high temperatures. A soluble HGI-III (rHGI) with the native N-terminus was produced using a pTWIN IMPACT™ purification system. Yield of rHGI was improved by introducing a trypsin sepharose affinity chromatography step resulting in ∼670 fold purification. The biochemical characteristics of rHGI point to its close similarity to seed HGI-III not only in its structure but also in its inhibitory characteristics toward bovine trypsin and chymotrypsin. The expression and purification strategy presented here promises to produce BBIs in their natural form for pharmacological and therapeutic use.


Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 1, 1980·Annual Review of Biochemistry·M Laskowski, I Kato
Feb 1, 1994·Bioscience, Biotechnology, and Biochemistry·S TeradaE Kimoto
May 1, 1996·Journal of Molecular Evolution·B PrakashL R Gowda
Oct 17, 2002·Journal of Agricultural and Food Chemistry·Franco M Lajolo, Maria Inés Genovese
Aug 1, 1953·The Biochemical Journal·M DIXON
May 5, 2004·The Journal of Biological Chemistry·Pradeep KumarLalitha R Gowda
Jun 5, 2004·Protein Expression and Purification·Alfonso ClementeClaire Domoney
Nov 20, 2004·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Y NaitoT Yoshikawa
Jan 13, 2006·Protein Expression and Purification·Madan Katragadda, John D Lambris
Jun 22, 2006·Current Protein & Peptide Science·Alfonso Clementea, C Domoney
Jun 7, 2007·Digestive Diseases and Sciences·Gary R LichtensteinJeffrey H Ware
Jun 19, 2007·Protein Expression and Purification·Gudrun VogtentanzBrian F Schmidt
Jul 5, 2008·Protein Expression and Purification·Roman S EsipovAnatoly I Miroshnikov
Feb 17, 2009·Protein Expression and Purification·Nanying CheChengcai An
Mar 17, 2010·Biochimica Et Biophysica Acta·Deepa G Muricken, Lalitha R Gowda
Mar 23, 2011·Current Protein & Peptide Science·Alfonso ClementeClaire Domoney
Jun 29, 2011·Protein Expression and Purification·Ting ChenYuanhao Qiao
Oct 2, 2012·Experimental and Molecular Pathology·Farinaz Safavi, Abdolmohamad Rostami

❮ Previous
Next ❯


Mar 25, 2014·Biotechnology Letters·Dongli Guan, Zhilei Chen
Apr 22, 2015·Journal of Food Science and Technology·Saroj Kumar Prasad, Manoj Kumar Singh
Jan 13, 2015·Journal of Industrial Microbiology & Biotechnology·Qing HeTian-jiao Li
Dec 21, 2014·Archives of Biochemistry and Biophysics·Vinod KumarLalitha R Gowda

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.