Production of human erythropoietin by chimeric chickens

Biochemical and Biophysical Research Communications
Daisuke KodamaS Iijima

Abstract

The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white-hEpo was comparable to that produced by recombinant CHO cells.

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Citations

Aug 5, 2009·Transgenic Research·Carlos Alberto PennoMasamichi Kamihira
Aug 25, 2012·Transgenic Research·Xiaojuan LiuXiaofang Liu
Sep 3, 2013·Biological & Pharmaceutical Bulletin·Osamu NakajimaReiko Teshima
Dec 21, 2013·Journal of Bioscience and Bioengineering·Yusuke KojimaShinji Iijima
Nov 15, 2011·Journal of Bioscience and Bioengineering·Daisuke KodamaShinji Iijima
Mar 17, 2010·Journal of Bioscience and Bioengineering·Makoto MotonoShinji Iijima
Nov 6, 2009·Current Opinion in Biotechnology·Yves Durocher, Michael Butler
May 12, 2009·Journal of Biotechnology·Masamichi KamihiraShinji Iijima
Jan 13, 2009·Journal of Bioscience and Bioengineering·Yoshinori KawabeMasamichi Kamihira
Oct 12, 2012·Annals of the New York Academy of Sciences·Tae Sub Park, Jae Yong Han
May 7, 2016·Journal of Bioscience and Bioengineering·Shunsuke KidaniShinji Iijima
Dec 17, 2014·Journal of Bioscience and Bioengineering·Yusuke KojimaShinji Iijima
Oct 12, 2018·Molecular Biotechnology·Rachel M WoodfintKichoon Lee
Dec 31, 2018·BMC Biotechnology·Lissa R HerronHelen M Sang
Dec 29, 2020·Journal of Bioscience and Bioengineering·Yusuke KojimaShinji Iijima
Oct 21, 2021·Biology of Reproduction·Nima DehdilaniHesam Dehghani

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