PMID: 6969366Dec 4, 1980Paper

Production of human hybridomas secreting antibodies to measles virus

Nature
C M CroceH Koprowski

Abstract

Monoclonal antibodies against a variety of antigens can be produced using techniques of somatic cell hybridization between cells of rodent myeloma lines and B cells derived from animals immunized against a given antigen. However, because of the monoclonal antibodies secreted by these hybridomas are of rodent origin, their use in human immunotherapy is limited. Thus the production of B-cell hybrids that secrete human monoclonal antibodies may be of considerable value. We have hybridized a hypoxanthine phosphoribosyl transferase (HPRT)-deficient human B-cell line derived from a patient suffering from multiple myeloma with peripheral lymphocytes obtained from a patient with subacute sclerosing panencephalitis (SSPE). These hybridomas were found to secrete human IgM specific for measles virus nucleocapsids.

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C M CroceH Koprowski
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W W HallP W Choppin

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Citations

Jan 1, 1983·Journal of Cancer Research and Clinical Oncology·J Zeuthen
Jan 1, 1982·The Journal of Membrane Biology·U Zimmermann, J Vienken
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Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M C GlassyI Royston

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