Abstract
A reverse hemolytic plaque assay has been developed which specifically detects secretion of human immune interferon (Hu IFN-gamma) at the single cell level. Unstimulated peripheral blood lymphocytes from healthy adult volunteers spontaneously secreted IFN-gamma. Stimulation of these cells with concanavalin A, phytohemagglutinin, or the UCHT1 monoclonal anti-human T cell antibody significantly increased the number of IFN-gamma-secreting cells. The cell producing IFN-gamma, both spontaneously and after UCHT1 antibody stimulation, is an OKT3+,4+,8-,HLA-DR-T lymphocyte as determined at the single cell level. Finally, cyclosporin A, a potent and selective immunosuppressive drug for T cells, strongly inhibited the secretion of IFN-gamma as assayed at the cell level. This IFN-gamma reverse hemolytic plaque assay has great potential for the further study of IFN-gamma both in physiological and pathological conditions.
References
Dec 23, 1978·Lancet·R Y CalneK Rolles
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E Svedmyr, M Jondal
Aug 1, 1977·The Journal of Experimental Medicine·D A Thorley-LawsonJ L Strominger
Oct 19, 1979·Science·P KungS F Schlossman
Oct 1, 1979·The Journal of Experimental Medicine·D PrimiJ W Goodman
Aug 1, 1976·European Journal of Immunology·E GronowiczF Melchers
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G A MolinaroS Dray
Mar 8, 1980·Lancet·J Nagington, J Gray
Jun 21, 1980·Lancet·D H CrawfordJ H Thompson
Aug 27, 1981·Nature·I NathanD W Golde
Oct 1, 1981·Journal of Virological Methods·M De LeyP De Somer
Jan 14, 1982·Nature·A G MorrisB A Askonas
Apr 1, 1982·The Journal of Experimental Medicine·J R KleinM J Bevan
Apr 1, 1982·The Journal of Experimental Medicine·J L McKimm-BreschkinJ F Miller
Apr 1, 1982·European Journal of Immunology·S LandolfoM M Simon
Mar 15, 1982·Cellular Immunology·R Palacios, C Fernandez
Jan 1, 1982·Immunological Reviews·D J White, R Y Calne
Jan 22, 1981·Nature·A G BirdS Britton
Jan 1, 1981·Immunobiology·R Palacios
Jan 1, 1982·Immunological Reviews·S Britton, R Palacios
Jan 1, 1981·Immunogenetics·R Palacios
Apr 1, 1981·European Journal of Immunology·P C Beverley, R E Callard
Citations
Jan 1, 1991·Medicinal Research Reviews·V St Georgiev
May 25, 1988·Journal of Immunological Methods·C CzerkinskyO Ouchterlony
Jun 24, 1992·Journal of Immunological Methods·I Nordström, B Ferrua
Jan 1, 1990·Pharmacology & Therapeutics·H A Young, K J Hardy
Feb 1, 1985·Antiviral Research·W WeimarJ Jeekel
Jan 1, 1986·International Journal of Immunopharmacology·M FujimotoT Tsujii
Jan 1, 1990·International Journal of Immunopharmacology·S SozzaniA Vecchi
Aug 1, 1994·Journal of Immunoassay·S AlkanH Towbin
Apr 1, 1988·Journal of Interferon Research·J F DowningM W Taylor
Mar 1, 1991·British Journal of Haematology·S Imashuku, S Hibi
Jan 1, 1989·Cancer Immunology, Immunotherapy : CII·K AhmedJ L Turk
Jan 1, 1991·Leukemia & Lymphoma·S ImashukuM Saito
Feb 1, 1991·Immunological Reviews·C CzerkinskyH P Ekre
Feb 1, 1985·Scandinavian Journal of Immunology·P J Converse, A D Hess
May 1, 1985·Scandinavian Journal of Immunology·P S SteegJ J Oppenheim
Nov 1, 1991·Cancer·A NumataT Tanabe
Aug 1, 1992·European Journal of Immunology·L Stitz
Jan 1, 1986·Microbiology and Immunology·K ShimokataH Tsunekawa
Jan 1, 1986·Microbiology and Immunology·I SugawaraA Fukuda
Mar 15, 1985·International Journal of Cancer. Journal International Du Cancer·M T BejaranoG Klein
Feb 1, 1985·European Journal of Immunology·R Palacios
Sep 1, 1989·European Journal of Immunology·B J SkidmoreJ M Chiller
Aug 1, 1984·Cellular Immunology·J E Kay, C R Benzie
Mar 1, 1986·Journal of Virology·S CharanR M Zinkernagel
Apr 1, 1986·Infection and Immunity·A W HüginR M Zinkernagel
Jan 1, 1991·Journal of Virology·L StitzK Frese