PMID: 9631525Jun 19, 1998Paper

Production of human lactoferrin in transgenic tobacco plants

Protein Expression and Purification
V SalmonB Mérot

Abstract

Production and characterization of human lactoferrin (hLf) in transgenic tobacco is reported. We have engineered two constructs containing either the native signal peptide from human lactoferrin or the signal peptide from sweet potato sporamin fused to human lactoferrin encoding cDNA. N-terminal sequences of rhLf purified from tobacco were identical to Lf from human milk for both constructs. The tobacco rhLf presents a molecular mass closely identical to native protein. Overall sugar composition shows the presence of plant specific xylose while sialic acid is absent. Binding parameters of the recombinant molecule to both Jurkat lymphoblastic T-cells or HT29-18-C1 enterocytes are similar to those of human lactoferrin isolated from milk.

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Citations

May 3, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Kenji TakaseKei-ichi Shimazaki
May 8, 2014·Cell Biology International·Alla I YemetsYaroslav B Blume
Jun 20, 2002·Journal of the American College of Nutrition·Bo Lönnerdal
Mar 7, 2006·Acta Biochimica Et Biophysica Sinica·Tao LiuXiang-Fu Wu
Aug 18, 2009·Transgenic Research·Sonia Gómez-GaleraPaul Christou
Feb 6, 2009·Biotechnology Progress·Carolina W RibeiroFlavio Henrique-Silva
Apr 20, 2006·Animal Biotechnology·Chunjiang ZhaoDinglian Zhang
Jul 19, 2003·European Journal of Biochemistry·Bénédicte Samyn-PetitPhilippe Delannoy
Mar 19, 2003·Journal of Chromatography. a·Deepa BalasubramaniamChenming Zhang
May 17, 2007·Journal of Agricultural and Food Chemistry·Celia ConesaMiguel Calvo
Jul 31, 2013·Journal of Agricultural and Food Chemistry·Dilip K LakshmanAmitava Mitra
Jul 7, 2009·Biotechnology Advances·Arun K Sharma, Manoj K Sharma

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