Production of hyaluronic acid by mutant strains of group C Streptococcus

Molecular Biotechnology
Marcela TlustáJana Jílková


This study addresses the influence of upstream region sequence on the strength of has operon promoter in highly encapsulated S. equi subsp. zooepidemicus (SEZ). For this purpose, seven different strains were constructed. Each strain carries a point mutation in one of the following positions upstream of the has promoter: -43, -44, -49, and -50 bp. To facilitate measuring of the recombinant promoter relative strength, ß-glucuronidase gene was used as a reporter gene. Three mutations located in positions -49 and -50: AT, GT, and AG, positively impacted has promoter strength when compared to the wild type sequence GG. Conversely, two other mutations: TG and TT, exhibited a slight inhibitory effect. Further, three different strains carrying chromosomal mutations in the has promoter region were constructed. In two cases, the has operon is under the control of a stronger promoter and in the third strain the has operon is controlled by a weaker promoter. The laboratory fermenter scale cultivations confirmed the increase of hyaluronan yields for SEZPhasAG and SEZPhas2G, resulting 116 and 105 %, respectively. As expected, the yield of the hyaluronic acid of SEZPhas2B strain fell to 41 %.


Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M R WesselsT J DiCesare
Jul 1, 1990·Molecular Microbiology·D A Morrison, B Jaurin
Sep 1, 1989·Equine Veterinary Journal·P M McCue, W D Wilson
Aug 4, 1995·The Journal of Biological Chemistry·D L Crater, I van de Rijn
Dec 1, 1994·International Journal of Biological Macromolecules·M O'ReganM Lansing
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·M R Wessels, M S Bronze
Nov 1, 1996·The Journal of Clinical Investigation·H M SchragerM R Wessels
May 30, 1997·The Journal of Biological Chemistry·P H WeigelM Tammi
Dec 31, 1997·Science·P L DeAngelisJ L Van Etten
Sep 9, 2000·Current Opinion in Cell Biology·J Y Lee, A P Spicer
Dec 16, 2004·Applied Microbiology and Biotechnology·Barrie Fong ChongLars K Nielsen
Jul 8, 2005·Applied and Environmental Microbiology·Bill WidnerSteve Brown
Oct 20, 2005·Journal of Bioscience and Bioengineering·Takashi Yamada, Takeru Kawasaki
Jan 16, 2007·Applied Microbiology and Biotechnology·Ján Krahulec, Jana Krahulcová
Aug 19, 2007·Biotechnology Progress·Liang-Jung Chien, Cheng-Kang Lee
Nov 6, 2007·The Journal of Biological Chemistry·Paul H Weigel, Paul L DeAngelis
Feb 12, 2008·Current Protocols in Molecular Biology·D D Moore
May 20, 2009·The Journal of Biological Chemistry·Wendy Yiting ChenLars Keld Nielsen

❮ Previous
Next ❯


Jun 25, 2014·Applied Microbiology and Biotechnology·Esteban MarcellinLars K Nielsen
Apr 30, 2021·Carbohydrate Polymers·Zhi-Yuan YaoJin-Song Shi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.