PMID: 9436534Jan 22, 1998Paper

Production of hyaluronidase by cultured human tumor cells

Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie
R VictorBertrand Delpech

Abstract

The presence of hyaluronidase was detected at pH 3.8 in eight out of twelve human cancer cell line culture media. Eight cell lines derived from primary tumours and four from metastases. In three culture media the enzymatic activity was lower than 0.035 pU/cell/h. In five others (in a hepatoma cell line and in four metastasis-derived cell lines) the activity was higher than 0.057 pU/cell/h. A tumour-derived fibroblast culture was negative. The optimal activity was observed at a pH comprised between 3.6 and 4. Salt inhibition of hyaluronidase was reversible. The enzyme was denaturated by a 10-min heating at 70 degrees C. The enzyme was not strictly specific for hyaluronan hydrolysis but also digested chondroitin sulfates. PH20, a spermatozoid protein that has homologies with the bee venom hyaluronidase, was not expressed by cell lines tested.

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Citations

Aug 29, 2003·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Catherine MaingonnatBertrand Delpech
Apr 28, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Hélène LenormandJean-Claude Vincent
Mar 30, 2001·The Journal of Biological Chemistry·V B LokeshwarB L Lokeshwar
Aug 9, 2005·Pathologie-biologie·Robert Stern
Dec 29, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Trias AstériouBrigitte Deschrevel
Mar 14, 2008·Matrix Biology : Journal of the International Society for Matrix Biology·Brigitte DeschrevelJean-Claude Vincent

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