PMID: 6262346Mar 1, 1981Paper

Production of hybrid cell lines secreting antibodies to herpes simplex virus type 2

Journal of Virological Methods
R A KillingtonS Bacchetti

Abstract

Following different immunization schedules we have produced 102 myeloma--spleen cell hybrid lines (30.8% of the total) secreting antibodies to HSV-2 infected cells. The highest yield of hybrids was derived from fusion of myeloma cells with spleen cells from mice sensitized by footpad injection of formalin-inactivated virus and boosted intravenously with the same antigen. To date, twelve of the antibody-producing hybrids have been cloned by limiting dilutions to generate over 100 monoclonal lines reacting specifically with HSV-infected but not with mock-infected cells. Several hybrid lines have also been injected into mice to generate tumors and high titers of anti-HSV-specific antibodies have been detected in ascitic fluids. All of the hybrids so far tested have retained the capacity to secrete anti-HSV antibodies after long-term in culture or repeated passages through mice. Antibodies reacting with cytoplasmic or nuclear antigens in infected cells as well as type-specific and type common antibodies have been detected.

References

Jan 1, 1976·Infection·M Majer
Feb 1, 1978·European Journal of Immunology·G KöhlerM J Shulman
Nov 1, 1977·The Journal of General Virology·R A KillingtonJ Mumford
Jul 1, 1979·The Journal of General Virology·E L HowesN A Mitchison
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H KoprowskiC M Croce
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Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT

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Citations

Jan 1, 1991·Archives of Virology·H Piiparinen, A Vaheri
Oct 1, 1984·European Journal of Clinical Microbiology·R A Polin
Dec 1, 1981·In Vitro·R H Kennett
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J R WandsD A Shafritz
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J R WandsK J Isselbacher
Sep 20, 2007·PloS One·Laurent GilletPhilip G Stevenson
Aug 1, 1984·Journal of Clinical Microbiology·B FrameM A Chernesky
Jul 1, 1982·Journal of Clinical Microbiology·N BalachandranW E Rawls
Oct 1, 1982·Journal of Virology·N BalachandranS Bacchetti
Aug 1, 1981·Journal of Virology·N BalachandranW E Rawls

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