PMID: 6262346Mar 1, 1981Paper

Production of hybrid cell lines secreting antibodies to herpes simplex virus type 2

Journal of Virological Methods
R A KillingtonS Bacchetti


Following different immunization schedules we have produced 102 myeloma--spleen cell hybrid lines (30.8% of the total) secreting antibodies to HSV-2 infected cells. The highest yield of hybrids was derived from fusion of myeloma cells with spleen cells from mice sensitized by footpad injection of formalin-inactivated virus and boosted intravenously with the same antigen. To date, twelve of the antibody-producing hybrids have been cloned by limiting dilutions to generate over 100 monoclonal lines reacting specifically with HSV-infected but not with mock-infected cells. Several hybrid lines have also been injected into mice to generate tumors and high titers of anti-HSV-specific antibodies have been detected in ascitic fluids. All of the hybrids so far tested have retained the capacity to secrete anti-HSV antibodies after long-term in culture or repeated passages through mice. Antibodies reacting with cytoplasmic or nuclear antigens in infected cells as well as type-specific and type common antibodies have been detected.


Jan 1, 1976·Infection·M Majer
Feb 1, 1978·European Journal of Immunology·G KöhlerM J Shulman
Nov 1, 1977·The Journal of General Virology·R A KillingtonJ Mumford
Jul 1, 1979·The Journal of General Virology·E L HowesN A Mitchison
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H KoprowskiC M Croce
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T J Wiktor, H Koprowski
Jul 1, 1976·European Journal of Immunology·G Köhler, C Milstein
Oct 1, 1968·Virology·Y BeckerI Sarov
Jan 1, 1974·Intervirology·P SethJ L Melnick
Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT

❮ Previous
Next ❯


Jan 1, 1991·Archives of Virology·H Piiparinen, A Vaheri
Oct 1, 1984·European Journal of Clinical Microbiology·R A Polin
Dec 1, 1981·In Vitro·R H Kennett
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J R WandsD A Shafritz
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J R WandsK J Isselbacher
Sep 20, 2007·PloS One·Laurent GilletPhilip G Stevenson
Aug 1, 1984·Journal of Clinical Microbiology·B FrameM A Chernesky
Jul 1, 1982·Journal of Clinical Microbiology·N BalachandranW E Rawls
Oct 1, 1982·Journal of Virology·N BalachandranS Bacchetti
Aug 1, 1981·Journal of Virology·N BalachandranW E Rawls

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.