Production of immunoglobulins in gingival tissue explant cultures from juvenile periodontitis patients

Journal of Periodontology
E R HallJ B Suzuki

Abstract

B lymphocytes and plasma cells are histologically observed in granulomatous periodontal tissues of juvenile periodontitis (JP) patients. Local immune processes may participate in protective or immunopathologic roles in the pathogenesis of this disease. An in vitro explant culture system was utilized to demonstrate the production of immunoglobulins by diseased JP tissues. Immunodiffusion studies using goat anti-human gamma, alpha, or mu chain serum revealed IgG to be the major immunoglobulin present in 92% of the day 1 supernatant fluids (SF) of the 47 JP gingival tissue explant cultures. IgA was present in 15% of the SF; however, no IgM was detected. Staph Protein A isolated 14C-labeled IgG from the SF, when allowed to react with goat anti-human gamma chain serum, formed lines of precipitation. Positive autoradiographs confirmed the biosynthesis of IgG by the explant cultures. The in vitro gingival tissue explant culture system described provides a useful model for the study of localized immunoglobulins produced by diseased tissues of JP patients.

References

Sep 1, 1987·Journal of Periodontal Research·W E Moore
May 1, 1986·Journal of Periodontology·S J SpindlerA D Costales
May 1, 1974·Journal of Periodontology·R J GencoS A Ellison
Jan 1, 1966·Journal of Periodontal Research·P Brandtzaeg
Jan 1, 1967·Journal of Periodontal Research·L Glavind, H Löe
Mar 1, 1980·Journal of Periodontal Research·E T LallyW P McArthur
Oct 1, 1984·Journal of Dental Research·J D MasonR A Yukna
Jan 1, 1981·Journal of Periodontology·T C WaldropW Killoy
Dec 1, 1963·Acta Odontologica Scandinavica·H LOE, J SILNESS
Jan 1, 1946·Journal of Periodontology·B GOTTLIEB
May 1, 1974·Journal of Periodontology·Robert J GencoSolon A Ellison

❮ Previous
Next ❯

Citations

Apr 11, 1992·Oral Microbiology and Immunology·J C BaumgartnerJ B Suzuki
Dec 1, 1994·Oral Microbiology and Immunology·E R HallW A Falkler
May 1, 1991·Journal of Periodontal Research·R R Ranney
Mar 1, 1996·Journal of Periodontology·David Engel
Jul 1, 1995·Clinical and Diagnostic Laboratory Immunology·T F MeillerC D Overholser

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.