PMID: 6170067Jul 1, 1981Paper

Production of immunologically active surface antigens of hepatitis B virus by Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
P MacKayK Murray

Abstract

Several plasmids have been constructed which direct the synthesis of hepatitis B virus surface antigens in Escherichia coli either as the native polypeptide or fused to other plasmid encoded polypeptides. When injected into rabbits, extracts from bacteria carrying some of these plasmids induced the synthesis of antibodies to the antigens even though the extracts did not give satisfactory positive results in radioimmunoassay for them. Either the NH2-terminal segment or the COOH-terminal segment of the surface antigens alone was sufficient to elicit the immune response, but antibodies against the two segments showed different specificities. The results emphasize the value of an in vivo assay for the presence of antigens in crude cell extracts and illustrate the feasibility of this type of screening with laboratory animals.

References

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Citations

Apr 26, 1996·Journal of Virological Methods·V Germaschewski, K Murray
Feb 1, 1985·Virus Research·K LundströmR F Pettersson
Jan 1, 1986·Biotechnology Advances·G G Khachatourians, C M Berezowsky
Oct 4, 1984·The New England Journal of Medicine·N C Engleberg, B I Eisenstein
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P K BhatnagarG N Vyas
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J L GerinR A Lerner
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F ArdeshirR T Reese
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S J Stahl, K Murray
Jun 11, 1983·Nucleic Acids Research·Y FujisawaY Sugino
Jul 2, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ding-Shinn Chen
Jan 1, 1981·Journal of Medical Virology·P MacKayK Murray
Oct 1, 1987·Journal of Medical Virology·K MurrayH Schellekens
Aug 31, 1989·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K MurrayP G Ashton-Rickardt
Oct 26, 2016·Biochemical Pharmacology·Christopher P Karch, Peter Burkhard

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