Production of infectious ferret hepatitis E virus in a human hepatocarcinoma cell line PLC/PRF/5

Virus Research
Tian-Cheng LiTakaji Wakita

Abstract

A strain of ferret hepatitis E virus (HEV), sF4370, isolated from an imported ferret was used to inoculate a human hepatocarcinoma cell line, PLC/PRF/5. The virus genome and capsid protein were detected in the cell culture supernatant. Immunofluorescence microscopy indicated that the capsid protein was located in the cytoplasm. The virus particles were purified from the culture supernatant by sucrose gradient ultracentrifugation. The capsid protein with molecular mass of ∼72 kDa was detected in fractions with density of 1.150-1.162 g/cm(3), and particles of ferret HEV was associated with cell membrane. The virus recovered from the supernatant was serially passaged with PLC/PRF/5 cells and had the ability to infect ferrets by oral inoculation, indicating that the ferret HEV grown in PLC/PRF/5 was infectious. The establishment of ferret HEV cell culture system might be useful to understand the life cycle, mechanism of infection and replication of ferret HEV.

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Citations

Dec 30, 2017·Transboundary and Emerging Diseases·H BaiT-C Li
Oct 5, 2018·Annual Review of Animal Biosciences·Scott P Kenney, Xiang-Jin Meng
May 8, 2018·Cold Spring Harbor Perspectives in Medicine·Tian-Cheng Li, Takaji Wakita
Aug 21, 2019·Nature Communications·Ankan Dutta ChowdhuryEnoch Y Park
May 5, 2017·Food and Environmental Virology·Nigel CookReimar Johne
Sep 30, 2019·Virus Research·Ibrahim M SayedMohamed A El-Mokhtar
Jan 18, 2019·Antiviral Research·Toni L MeisterEike Steinmann

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