PMID: 7545484Feb 1, 1995Paper

Production of insulin-like growth factor binding proteins (IGFBPs) by human endometrial stromal cell is stimulated by the presence of embryos

Journal of Assisted Reproduction and Genetics
H C LiuZ Rosenwaks

Abstract

To identify IGFBPs among endometrial secretory products and study their role in implantation and early embryo development. Two-cell CB6F1 mouse embryos were cultured alone or with human endometrial stromal cells in RPMI 1640 plus 10% fetal calf serum (FCS) with or without addition of IGF-I (20 micrograms/ml), IGF receptor antibody (0.1 microgram/ml), progesterone (P) (20 ng/ml) and relaxin (R) (20 micrograms/ml). On the designated day, the medium was changed to protein-free RPMI and incubated for 16 h. Both conditioned medium and conditioned protein-free medium were then collected for protein analysis and immunoradiometric assay. Cells were fixed with 4% paraformaldehyde for immunohistochemical staining. IGFBP1 (31 kDa), IGFBP2 (36 kDa), IGFBP3 (45 kDa and 50 kDa) and an unknown IGFBP (25 kDa) were identified in conditioned medium of human endometrial stromal cells cultured alone or cocultured with mouse embryos. Secretion of IGFBPs by endometrial stromal cells was stimulated in the presence of mouse embryos as well as by P and R. IGFBP3 appears to be more responsive to embryonic signals. On the other hand, the secretion of IGFBP1 was greatly stimulated by P and R. Immunolocalization revealed that all three BPs were present in ...Continue Reading

References

Feb 1, 1992·Human Reproduction·Y M LaiZ Rosenwaks
Jul 1, 1987·Molecular Endocrinology·L J MurphyH G Friesen
Mar 1, 1988·Endocrinology·M B Harvey, P L Kaye
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D J Knauer, G L Smith
Sep 1, 1994·Biology of Reproduction·R A BoomsmaH G Verhage
Apr 1, 1994·Journal of Assisted Reproduction and Genetics·H C LiuZ Rosenwaks

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Citations

Dec 16, 1998·Fertility and Sterility·L I BarmatZ Rosenwaks
Jun 18, 2004·Reproductive Biology and Endocrinology : RB&E·Eric S Hayes
Jul 27, 1999·American Journal of Human Genetics·C M BrewerD R FitzPatrick
May 20, 2000·Biology of Reproduction·A HerrlerF Stewart
Jul 8, 2011·American Journal of Reproductive Immunology : AJRI·Najwa A RashidKristina Gemzell-Danielsson
Sep 25, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Özgecan Korkmaz AğaoğluSelim Aslan
Sep 27, 2018·Reproduction, Fertility, and Development·Iside ScaravaggiStefan Bauersachs
Dec 29, 2020·Journal of Reproductive Immunology·Fengrun SunMeirong Du

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