Production of Japanese Encephalitis Virus Antigens in Plants Using Bamboo Mosaic Virus-Based Vector

Frontiers in Microbiology
Tsung-Hsien ChenYau-Heiu Hsu

Abstract

Japanese encephalitis virus (JEV) is among the major threats to public health in Asia. For disease control and prevention, the efficient production of safe and effective vaccines against JEV is in urgent need. In this study, we produced a plant-made JEV vaccine candidate using a chimeric virus particle (CVP) strategy based on bamboo mosaic virus (BaMV) for epitope presentation. The chimeric virus, designated BJ2A, was constructed by fusing JEV envelope protein domain III (EDIII) at the N-terminus of BaMV coat protein, with an insertion of the foot-and-mouth disease virus 2A peptide to facilitate the production of both unfused and epitope-presenting for efficient assembly of the CVP vaccine candidate. The strategy allowed stable maintenance of the fusion construct over long-term serial passages in plants. Immuno-electron microscopy examination and immunization assays revealed that BJ2A is able to present the EDIII epitope on the surface of the CVPs, which stimulated effective neutralizing antibodies against JEV infection in mice. This study demonstrates the efficient production of an effective CVP vaccine candidate against JEV in plants by the BaMV-based epitope presentation system.

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Citations

Jun 12, 2018·Plant Biotechnology Journal·Emmanuel MargolinAnn E Meyers
Aug 11, 2018·Reviews in Medical Virology·Garry A Luke, Martin D Ryan
Mar 4, 2020·Viruses·Ina Balke, Andris Zeltins
Jan 5, 2021·Applied Microbiology and Biotechnology·Peter AbrahamianRosemarie W Hammond

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Methods Mentioned

BETA
transgenic
PCR
electrophoresis
Enzyme-Linked Immunosorbent Assay
ELISA
transmission electron microscopy
electron microscopy

Software Mentioned

MagnICON

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